https://www.selleckchem.com/products/Trichostatin-A.html This study aimed to investigate the effects of 17β-estradiol and estrogen receptors (ERs) in U2OS cells. Osteosarcoma U2OS cells were divided into six groups, and cell proliferation was determined using the cell counting kit-8 growth test. Furthermore, U2OS cell migration and invasion were examined by cell scratch test and Transwell invasion assays, respectively. At 48 h of 17β-estradiol exposure, U2OS cell viability decreased ( <0.001); however, ERα siRNA and ERβ siR-NAs significantly increased cell viability ( <0.01). Considering the cell positions at 0 h, the cell migration distance at 24 h significantly reduced in the presence of 17β-estradiol ( <0.001); however, ERα and ERβ siRNAs significantly increased cell migration distance ( <0.01). The number of invasive cells significantly decreased upon exposure to 17β-estradiol ( <0.001); however, ERα and ERβ siRNAs significantly increased the number of invasive cells ( <0.01 and <0.05, respectively). 17β-estradiol exhibited significant anti-tumor effects on U2OS cells that were mediated by ERs and reduced cell proliferation, migration, and invasion. 17β-estradiol exhibited significant anti-tumor effects on U2OS cells that were mediated by ERs and reduced cell proliferation, migration, and invasion.Macrophages have the potential to re-programing tumor cells in the tumor microenvironments. Thus we investigated anti-cancer effects of M1-polarized macrophages by lipopolysaccharide (LPS) on the physiological properties of human prostate cancer PC-3 cells. To identify communications with immune cells and tumor cells, we performed in-direct way by using conditioned-media (CM) and analyzed tumor properties via quantitative polymerase chain reaction, enzyme-linked immunosorbent assay and western blot and flow cytometry. CM of M1-polarized macrophages induced apoptotic cell death in PC-3 cells, and it surprisingly suppressed tumor parameters including epithelial to mesenc