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https://www.selleckchem.com/products/Y-27632.html Dietary methionine restriction is associated with improved health outcomes and an increase in lifespan in animal models. We have previously shown that an increase in dietary methionine induces alteration in the intestinal microbiome. The composition of the intestinal microbiota is a determinant of health and we, therefore, hypothesized that dietary methionine restriction would also induce changes in the murine microbiome. After one month on a methionine-restricted diet, five-month-old male and female C57BL/6 mice had decreased levels of serum methionine, without changes in body weight. We identified a decrease in the hepatic methylation status of animals fed a methionine-restricted diet compared to controls. This decrease was not associated with changes in DNA or protein methylation in the liver. In males, we saw an increase in families Bacteroidaceae and Verrucoccaceae (mostly A. mucinophila) and a decrease in Rumminococcaceae in animals fed a methionine-restricted diet compared to controls. In females, Bacteroidales family S24-7 was increased two-fold, while families Bacteroidaceae, Verrucoccaceae, Rumminococcaceae, and Rikenellaceae were decreased compared to controls. In summary, feeding a methionine-restricted diet for one month was associated with significant and sex-specific changes in the intestinal microbiome.OCRA (OCcupational Repetitive Action) is currently one of the most widespread procedures for assessing biomechanical risks related to upper limb repetitive movements. Frequency factor of the technical actions represents one of the OCRA elements. Actually, the frequency factor computation is based on workcycle video analysis, which is time-consuming and may lead to up to 30% of intra-operator variability. This paper aims at proposing an innovative procedure for the automatic counting of dynamic technical actions on the basis of inertial data. More specifically, a threshold-based algorithm was tested in f

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