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as the amount of LJE increased. In summary, LJE had no negative effects on lactation performance but helped to alleviate heat stress by improving antioxidant status and promoting endocrine and immune functions. Supplementation with LJE at 28 g/d is recommended for lactating dairy cows experiencing heat stress during hot summers.There is ongoing debate regarding whether critically important antimicrobials (CIA) should be used to treat infections in food-producing animals. In this systematic review, we determined whether CIA and non-CIA have comparable efficacy to treat nonsevere bovine clinical mastitis caused by the most commonly reported bacteria that cause mastitis worldwide. We screened CAB Abstracts, Web of Science, MEDLINE, Scopus, and PubMed for original epidemiological studies that assessed pathogen-specific bacteriological cure rates of antimicrobials used to treat nonsevere clinical mastitis in lactating dairy cows. Network models were fit using risk ratios of bacteriological cure as outcome. A total of 30 studies met inclusion criteria. Comparisons of cure rates demonstrated that CIA and non-CIA had comparable efficacy for treatment of nonsevere clinical mastitis in dairy cattle. Additionally, for cows with nonsevere clinical mastitis caused by Escherichia coli and Klebsiella spp., bacteriological cure rates were comparable for treated versus untreated cows; therefore, there was no evidence to justify treatment of these cases with CIA. Our findings supported that CIA in general are not necessary for treating nonsevere clinical mastitis in dairy cattle, the disease that accounts for the majority of antimicrobial usage in dairy herds worldwide. Furthermore, our findings support initiatives to reduce or eliminate use of CIA in dairy herds.During inflammation of the mammary gland, the blood-milk barrier, which is predominantly composed of mammary epithelial cells, loses its integrity and gradients between blood and milk cannot be maintained. Nonsteroidal anti-inflammatory drugs (NSAID) are commonly used systemically in combination with local administration of antimicrobials in mastitis treatments of dairy cows to improve the well-being of the cow during the disease. However, the knowledge about their effects on the blood-milk barrier is low. This study aimed to investigate effects of different NSAID, with different selectivity of cyclooxygenase-inhibition, on the transepithelial electrical resistance (TEER) and capacitance, cell viability, and expression of tumor necrosis factor α of bovine mammary epithelial barriers in vitro. Primary mammary epithelial cells of 3 different cows were challenged with lipopolysaccharide (LPS) from Escherichia coli with or without addition of ketoprofen (1.25 mg/mL or 4 mM), flunixin meglumine (1.0 mg/mL or 4 mM)c slowed down the recovery (return to control level after 24 h). In conclusion, NSAID do not prevent the mammary epithelial barrier opening by LPS; however, ketoprofen, flunixin meglumine, and meloxicam obviously support the re-establishment of the barrier integrity. Used in mastitis therapy at an optimized dosage the tested NSAID would likely support the recovery of milk composition. However, an overdose of NSAID would likely cause tissue irritation and in turn, a delayed recovery of the barrier permeability.The widespread use of sexed semen on US dairy cows and heifers has led to an excess of replacement heifers' calves, and the sale prices for those calves are much lower than in the past. Females not selected to produce the next generation of replacement heifers are increasingly being bred to beef bulls to produce crossbred calves for beef production. The purpose of this study was to investigate the use of beef service sires bred to dairy cows and heifers and to provide a tool for dairy producers to evaluate beef service sires' conception. Sire conception rate (SCR) is a phenotypic evaluation of service sire fertility that is routinely calculated for US dairy bulls. A total of 268,174 breedings were available, which included 36 recognized beef breeds and 7 dairy breeds. Most of the beef-on-dairy inseminations (95.4%) were to Angus (AN) bulls. Because of the limited number of records among other breeds, we restricted our final evaluations to AN service sires bred to Holstein (HO) cows. Service-sire inbreeding antion rate. https://www.selleckchem.com/products/AZD2281(Olaparib).html Mean service number was 1.92 and 2.87 for HO heifers and 2.13 and 3.04 for HO cows mated to HO and AN sires, respectively. Mating dairy cows and heifers to beef bulls may be profitable if calf prices are higher, fertility is improved, or if practices such as sexed semen, genomic testing, and improved cow productive life allow herd owners to produce both higher quality dairy replacement and increased income from market calves.We hypothesized that dairy cows fed oscillating metabolizable protein (MP) and crude protein (CP) concentrations on a 24-h frequency for a diet formulated to be below MP requirements would use N more efficiently (i.e., increased milk protein yields and less manure N) without increasing mobilization of body protein stores than would cows fed the same deficient MP diet continuously, although both treatments would on average have equal MP concentrations. In a randomized block design, 30 Holstein cows (119 ± 21 d in milk; 667 ± 69 kg of body weight) were blocked according to milk yield within a parity (3 primiparous and 7 multiparous blocks) and fed 1 of 3 treatments (1) diet with 16.2% CP (109% of MP requirements) fed continuously (109MP), (2) diet with 14.1% CP (95% of MP requirements) fed continuously (95MP), or (3) diets oscillating on a 24-h cycle from the 109MP diet and a diet with 11.9% CP (∼78% of MP requirements) such that average CP and MP concentration would be the same as 95MP (OSC). Dry matter intakeas similar for major nutrients across treatments except for CP, which was greater for 109MP (65.2%) and OSC (65.3%) compared with 95MP (61.7%). Compared with 95MP, OSC did not increase milk N relative to N intake (averaged 0.35 g of milk N/g of N intake) or N balance; however, urinary N output was increased for OSC versus 95MP (0.32 vs. 0.24 g of urine N/g of N intake). Body composition estimated using urea dilution was similar across treatments, and all cows accreted lipid and energy during the trial. Empty body CP did not change over the 50-d treatment period. Overall, greater CP digestion, urinary N excretion, and MUN concentrations with lesser N intake and similar milk N outputs for OSC compared with 95MP suggests that the lower energy intake by OSC cows may have limited potential responses to altered N metabolism.
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