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https://www.selleckchem.com/products/sbi-115.html CypB silencing/knockout leads to Slug, but not Snail, down-regulation. CypB's support of Slug expression depends on its endoplasmic reticulum location, where it interacts with calreticulin, a calcium-buffering chaperone related to Slug expression. Since CypB silencing reduces ionomycin-induced calcium release and Slug up-regulation, we suggest that Slug expression may rely on CypB modulation of calreticulin-dependent calcium signaling. In conclusion, this work uncovers new roles for CypA and CypB in modulating TECs plasticity and identifies CypB as a druggable target potentially relevant in promoting kidney repair. © The Author(s) 2020. Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS.BACKGROUND Autologous fat is known for a reliable and natural safety profile, but complications do occur - even serious vascular adverse events. OBJECTIVES To examine duplex-ultrasound (DUS) imaging for the harvesting and subsequent facial implantation of autologous fat tissue. METHODS All patients underwent lipofilling treatment of the temporal fosse of the face. DUS examination was performed for pre-procedural vascular mapping and imaging of previously injected (permanent) fillers. In addition, the injection of autologous fat was performed DUS-guided. RESULTS Twenty patients (all female; mean age, 57.9 years; range, 35-64 years). DUS examination showed that sixteen out of the 20 patients (80%) had been injected with resorbable or non-resorbable fillers elsewhere in the past. The temporal artery could be visualized and avoided in all cases. An average of 1.1 cc of autologous fat was injected in the temporal fossa per side. One case of edema and nodules was described, but no other adverse events were reported. CONCLUSIONS The use of duplex-ultrasound can add valuable information to a lipofilling procedure. Its use should be considered as integral part of a safe lipofilling treatment. ©

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