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Predictive abilities of different diagnostic thresholds were undertaken using positive/negative predictive values, sensitivity/specificity statistics and logistic regressions. RESULTS At Week 9, 15 participants (7%) were identified as experiencing ICD-11 PTSD, compared to 23 (11%) experiencing ICD-10 PTSD. There was no significant difference in comorbidity rates between ICD-10 and ICD-11 PTSD diagnoses. Ninety per cent of participants with ICD-11 PTSD also met criteria for at least one CPTSD feature. Five participants met full CPTSD criteria. CONCLUSIONS Reduced prevalence of PTSD associated with the use of ICD-11 criteria is likely to reduce identification of PTSD relative to using ICD-10 criteria but not relative to DSM-4 and DSM-5 criteria. Diagnosis of CPTSD is likely to be infrequent following single-incident trauma. © 2020 The Authors. Journal of Child Psychology and Psychiatry published by John Wiley & Sons Ltd on behalf of Association for Child and Adolescent Mental Health.The use of alternative light sources (ALS) in bloodstain analysis has focused on dried (whole) blood, while information on detection of blood serum is lacking. Serum detection by ALS could provide valuable information at a crime scene, as serum may become separated from blood during clotting and cast off, especially in cases where the victim is moved. Additionally, a perpetrator may concentrate on the removal/scouring of dried blood with small amounts of serum going unnoticed, as it dries relatively clear on certain objects. In this report, the detection of human blood serum was evaluated using ultraviolet (UV) light at two different wavelengths. These results show that ultraviolet (UV) at 365 nm (UV365) was effective in the detection of even small amounts of blood plasma and serum, compared with UV at 395 nm, which was not. UV365 was also found to be useful in distinguishing blood imprints from clotting blood which had been transferred to material versus blood that had been added directly. Taken together, these results demonstrate that UV365 may be utilized as a simple, nondestructive method for blood serum detection. © 2020 The Authors. Journal of Forensic Sciences published by Wiley Periodicals LLC on behalf of American Academy of Forensic Sciences.The field of Biochemical Engineering is vast. From its historical origins in the microbial production of antibiotics in the 1940's, today's Biochemical Engineer may contribute to advances in a wide range of technical areas. The recent Biochemical and Molecular Engineering XXI conference focused on "The Next Generation of Biochemical and Molecular Engineering The role of emerging technologies in tomorrow's products and processes". Based on topical discussions at this conference, this Perspective synthesizes one vision on where investment in research areas is needed for biotechnology to continue contributing to some of the world's grand challenges. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.As an important bulk chemical, benzoic acid is currently manufactured from non-renewable feedstocks under harsh conditions. Although there are natural pathways for biosynthesis of benzoic acid, they are often inefficient and subjected to complex regulation. Here we develop a non-natural enzyme cascade to efficiently produce benzoic acid from styrene or biogenic ʟ-phenylalanine under mild conditions. By using a modular approach, two whole-cell catalysts E. coli LZ305 and LZ325 are engineered for co-expressing seven and nine enzymes for production of 133-146 mM benzoic acid (16.2-17.8 g/Laq ) with 88-97% conversion via seven- and nine-step cascade biotransformation of styrene and ʟ-phenylalanine, respectively. The seven-step cascade represents a formal high-yielding biocatalytic oxidative cleavage of styrene, and the nine-step cascade showcases the high efficiency of extended non-natural enzyme cascades. Moreover, to achieve benzoic acid production directly from low-cost renewable glycerol, a novel coupled fermentation-biotransformation process was developed by integration of fermentative production of ʟ-phenylalanine with in situ biotransformation to give 63-70 mM benzoic acid (7.6-8.6 g/Laq ), which is around 20 times higher than the reported value via a natural pathway. The coupled fermentation-biotransformation process could be generally applicable to microbial production of growth-inhibitory or toxic chemicals in high concentrations. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.The Seattle, Washington area was ground zero for coronavirus disease 2019 (COVID-19). https://www.selleckchem.com/products/ots964.html Its initial emergence in a skilled nursing facility (SNF) not only highlighted the vulnerability of its patients and residents, but also the limited clinical support that led to national headlines. Furthermore, the coronavirus pandemic heightened the need for improved collaboration among healthcare organizations and local and state public health. The University of Washington Medicine's Post-Acute Care Network developed a three phase approach, Initial, Delayed and Surge Phases, to help slow the spread of the disease, support local area SNFs from becoming overwhelmed when inundated with COVID-19 cases or persons under investigation, and help decrease the burden on area hospitals, clinics, and emergency medical services. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.OBJECTIVE Cutaneous smooth muscle tumors represent a rare group of cutaneous lesions including piloleiomyoma, angioleiomyoma, genital leiomyoma, smooth muscle hamartoma, and leiomyosarcoma. In this study, we aimed to evaluate the clinical and pathological characteristics of CSMTs, focusing on the rare and unspecified histological features. MATERIAL AND METHOD The clinical, demographic and histological findings of the patients with CSMTs were reviewed and evaluated retrospectively. The histopathological sections were re-evaluated for all cases. RESULTS A total of 32 patients with CSMTs were enrolled. The majority were female (n=20). The most common tumor diagnosed was angioleiomyoma (n=19, 59.4%) followed by piloleiomyoma (n=8, 25%), smooth muscle hamartoma (n=2, 6.3%), leiomyosarcoma (n=2, 6.3%), and genital leiomyoma (n=1, 3%). Five lesions were painful and only 3 specimens were submitted with the preliminary diagnosis of a cutaneous smooth muscle tumor. CONCLUSION There are very few studies investigating both clinical and histological characteristics of CSMTs in detail.
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