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his is a retrospective level 2 study. The correction of the extra-articular deformity by intra-articular bone resections performed at the time of a total knee arthroplasty is indicated if the resections do not affect the femoral or tibial insertions of the collateral ligaments of the knee, and is the treatment option to avoid performing an osteotomy to correct de limb axis. This method can be applied to angular deformities up to 20° in the femur and up to 30° in the tibia. Through this technique we have achieved good results, after more than 10 years of follow up, in 27 of the 29 patients treated.This is a retrospective level 2 study. The study investigated the chemical composition, antioxidant content, and antioxidant activity of honey (TVH) and essential oil (OVEO) and their mixture effect on carbon tetrachloride (CCl )-induced toxicity. The study conducted physicochemical characterization and chemical analysis of TVH and OVEO with the use of gas chromatography-mass spectrometry and high-performance liquid chromatograph (HPLC). The antioxidant activity of TVH and OVEO was done with the use of 1,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. The study used nine groups of rats to investigate the effect of TVH, OVEO, and a mixture of TVH and OVEO (HEM) on CCl -induced toxicity. Intraperitoneal injection of CCl (1 mL/100 g) was used to induce toxicity. The doses of TVH and OVEO were 1 mg/kg.b.wt, and 50 mg/kg.b.wt, respectively. HEM contains TVH (1 mg/kg.b.wt) and OVEO (50 mg/kg.b.wt). TVH has a high content of phenols, flavonoids, and flavanols. HPLC analysis showed that TVH contains, for the 1 time, epicatechin gallate, and at a high concentration. OVEO includes a high percentage of carvacrol and thymol. With the use of DPPH, OVEO was more potent than TVH. CCl caused significant liver and kidney damage and lipid disorders, which were alleviated by HVT, OVEO, and HEM. HVT was more potent than OVEO (p<0.05), and HEM was more potent than HVT and OVEO (p<0.05). The study identified high content of epicatechin gallate for the 1 time in TVH, and OVEO contains a high percentage of thymol and carvacrol. Epicatechin gallate might be useful as a marker for TVH. Mixing OVEO and TVH significantly potentiated their protection against CCl -induced liver and kidney toxicity. The study identified high content of epicatechin gallate for the 1st time in TVH, and OVEO contains a high percentage of thymol and carvacrol. Epicatechin gallate might be useful as a marker for TVH. Mixing OVEO and TVH significantly potentiated their protection against CCl4-induced liver and kidney toxicity. In recent times, non-aureus staphylococci (NAS) have emerged as the major organisms isolated from mastitis cases in dairy animals, with a predominance of and . As compared to , much less is known about the molecular types or the spatiotemporal epidemiology of these NAS species. In the present study, randomly amplified polymorphic DNA (RAPD) was employed to detect genetic polymorphisms, intraspecies diversity, and epidemiology of strains (n=37) isolated from bovine and bubaline mastitis cases in the state of Karnataka. Thirty-seven isolates (14 from bovines and 23 from bubaline) isolated from subclinical mastitis cases, from organized and unorganized sectors, were subjected to RAPD typing. https://www.selleckchem.com/products/ly-411575.html Further, methicillin resistance was determined by cefoxitin disk diffusion method. The amplified DNA fragments ranged from 150 to 3000 base pairs and yielded several RAPD profiles. Further analysis using Digital Image Correlation Engine correlation coefficient and UPGMA method showed that the 37 isolates could be classified into 12 distinct RAPD types (A to L) at 62% similarity ( =0.889). Four of the most predominant RAPD types, B, A, C, and E, in that order, and together, represented 65% of the isolates. High diversity was observed among the isolates both within farms and between geographic locations. Most of the isolates exhibited methicillin resistance. This is the first such report from India. In the absence of defined multilocus sequence type protocols or sufficient sequences available in the public domain, RAPD can be employed to determine genetic diversity of isolates. In the absence of defined multilocus sequence type protocols or sufficient sequences available in the public domain, RAPD can be employed to determine genetic diversity of S. chromogenes isolates. Newcastle disease viruses (NDVs) are frequently acquired from all ages and types of bird species. In general, ducks are considered as potential reservoirs for different genotypes of NDV and are resistant even to velogenic NDV strains. This research was conducted to genotypically and phylogenetically characterize NDV isolates collected from unvaccinated ducks from Indonesia. A total of 200 samples were collected through cloacal swabs and were inoculated in the allantoic sacs of 8-day-old specific pathogen-free eggs. Hemagglutination (HA) activity was analyzed through a HA test, and isolated viruses were characterized by reverse transcription-polymerase chain reaction targeting the complete fusion ( )-gene of NDV using three primer sets. One primer set was specific for the F protein cleavage site sequences of velogenic, mesogenic, and lentogenic NDV strains. The results demonstrated that three isolates (NDV/Duck/B104/19, NDV/Duck/B125/19, and NDV/Duck/BK43/19) belonged to genotype VII and one (NDV/Duck/TD19/19) to genotype VI. Other isolates (NDV/Duck/A74/19 and NDV/Duck/M147/19) belonged to genotype II Class II. Based on the F protein cleavage site and the pathogenicity tests, two isolates (NDV/Duck/B104/19 and NDV/Duck/B125/19) were categorized as velogenic viruses and four (NDV/Duck/BK43/19, NDV/Duck/TD19/19, NDV/Duck/A74/19, and NDV/Duck/M147/19) as lentogenic viruses. The results indicate that NDVs from unvaccinated ducks from Indonesia carry various genotypes and pathotypes of NDVs; therefore, these viruses are still circulating in the environment and might pose a risk of Newcastle disease outbreak. The results indicate that NDVs from unvaccinated ducks from Indonesia carry various genotypes and pathotypes of NDVs; therefore, these viruses are still circulating in the environment and might pose a risk of Newcastle disease outbreak.
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