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https://www.selleckchem.com/products/17-AAG(Geldanamycin).html MiR-155-5p is a key oncogenic microRNA that maintains immune homeostasis and mediates crosstalk between inflammation and tumorigenesis. High expression of PD-L1 also plays an important role in immune tolerance in tumors. The aim of this study was to explore the relationship between miR-155-5p and PD-L1 in lung adenocarcinoma (LUAD) cells A549 and H1650. The expression levels of miR-155-5p and PD-L1 in LUAD patients were detected by RT-qPCR, and mimics of miR-155-5p were used to model increased expression in A549 or H1650 cells. After 24 h, we measured levels of PD-L1 by RT-qPCR, western blot and flow cytometry. In addition, we identified two sites in the PD-L1 3'-UTR (5'-AGCAUUA-3' and 5'-GCAUUAA-3') which can be bound by miR-155-5p using TargetScan. Compared with normal tissue, miR-155-5p was overexpressed in tumor tissue (P = 0.0456), while the expression of PD-L1 was not significantly different (P = 0.1349). The expression levels of miR-155-5p and PD-L1 were negatively correlated (r = -0.6409, P = 0.0459 and r = -0.7544, P = 0.0117). Exogenous overexpression of miR-155-5p decreased the mRNA, total protein and membrane protein expression levels of PD-L1 both in A549 and H1650 cells (P less then 0.05). Taken together, our data suggest that miR-155-5p may suppress the expression of PD-L1 in LUAD. This article is protected by copyright. All rights reserved.INTRODUCTION Lupus nephritis (LN) is a common complication of systemic lupus erythematosus (SLE), which is a chronic autoimmune disease. However, the detailed mechanisms underlying this disorder have remained unclear. Alpha2-antiplasmin (α2AP) is known to perform various functions, such as plasmin inhibition and cytokine production, and to be associated with immune and inflammatory responses. METHODS We investigated the roles of α2AP in the pathogenesis of LN using a pristane-induced lupus mouse model. RESULTS The levels of plasmin-α2AP complex and α2AP

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