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https://www.selleckchem.com/products/ljh685.html Additionally, vacuolar amino acid transporters, the urea cycle, and urea hydrolysis genes were upregulated, indicating N recycling within the cells under N deficiency. Our study indicates that P. shikokuense copes with N deficiency by economizing nitrogen use and adopting multiple strategies to maximize N acquisition and reuse while maintaining carbon fixation. The remarkable low N adaptability may confer competitive advantages to P. shikokuense for forming harmful blooms in DIN-limited environments.The hyper-oligotrophic waters of the South Pacific Subtropical Gyre (SPSG) and the productive coastal Humboldt Current System (HCS) constitute an extreme nutrient gradient in the eastern South Pacific Ocean. Rich and dense fouling communities are known from floating objects in the HCS, but they have not been studied in the SPSG and it is not known which factors are influencing their richness and abundance. Here we present the first extensive study of rafting by marine invertebrates on floating anthropogenic debris in the eastern SPSG. We compared the effect of 9 raft-related categorical predictors on epibiont richness and fouling cover. Raft complexity was the most important predictor of richness. Fouling was dominated by thin crusts and biofilms, with more advanced communities only observed on few items. Fouling cover could not be predicted by any of the categorical factors tested. However, when tested as continuous predictors, raft volume and surface area were significantly correlated with both cover and richness. The most frequently encountered epibionts were common pelagic rafters, particularly Lepas spp., Planes spp., and Jellyella spp. Low fouling cover suggests that the SPSG's hyper-oligotrophic conditions strongly limit fouling growth, while the low frequency of coastal taxa points to the HCS/SPSG nutrient gradient acting as a filter for such organisms.Changing environments of temperature, precipitation and moistur

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