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Biopolymers have generated mounting interest among researchers and industrialists over the recent past. Rising consciousness on the use of eco-friendly materials as green alternatives for fossil-based biopolymers has shifted the research focus towards biopolymers. Advances in technologies have opened up new windows of opportunities to explore the potential of biopolymers. https://www.selleckchem.com/products/VX-770.html In this context, this review presents a critique on applications of biopolymers in relation to antioxidant and antimicrobial activities. Some biopolymers are reported to contain inherent antioxidant and antimicrobial properties, whereas, some biopolymers, which do not possess such inherent properties, are used as carriers for other biopolymers or additives having these properties. Modifications are often performed in order to improve the properties of biopolymers to suit them for different applications. This review aims at presenting an overview on recent advances in the use of biopolymers with special reference to their antioxidant and antimicrobial applications in various fields.Olive (Olea europaea L.) leaves and tea (Camellia sinensis) are rich sources of bioactive compounds, especially polyphenols. Our previous studies have evidenced the potential use of Saccharomyces cerevisiae as a natural delivery system for these antioxidants and a means to improve their bioaccessibility in the human gut. In the present work, the antiproliferative effect of green tea (GT), black tea (BT) and olive leaves (OL) infusions and suspensions of S. cerevisiae were evaluated, for the first time, in human colon cancer cells (Caco-2) after biosorption and in vitro gastrointestinal digestion. The bioaccessible fractions (BF) were not overtly cytotoxic, not affecting cell viability. ROS and mitochondrial membrane potential changes (Δψm) values were reduced compared with control cells. Moreover, all the BF after biosorption induced a significant (p less then 0.05) increase in cell proportions in S-phase. The arrest of the cell cycle was reversible without induction of apoptosis, suggesting that the biosorbed phenolics in both infusions and suspensions act as cytostatic agents.The prevalence of constipation increases rapidly with the increased pressure of some people's life, which seriously affects the quality of life in related patients. In this study, the improvement of functional constipation by Durio zibethinus Murr rind polysaccharide (DZMP) and the effects of DZMP on intestinal microbiota were investigated in a constipation model of Sprague-Dawley (SD) rats established by loperamide hydrochloride. Results showed that DZMP at 200 mg/kg could significantly (P less then 0.05) increase the intestinal transit rate, motilin, gastrin, substance P levels and concentration of short-chain fatty acids (SCFAs), reduce the somatostatin levels and improve the gastrointestinal peristalsis of rats. Sequencing showed that the Lachnospiraceae-NK4A136-group in the rats given 200 mg/kg DZMP (16.07%) was significantly higher than that of the model group (10.13%), while the Desulfovibrio was lower (2.99%) than that of the model group (4.19%). Principal co-ordinates analysis (PcoA) revealed a significant difference in intestinal microbiota composition between the model group and the high-dose DZMP group (200 mg/kg). The results demonstrated that DZMP has a regulatory effect of treating functional constipation and regulating intestinal flora in rats.The presence of white colony-forming yeast (WCFY) on kimchi surfaces indicates a reduction in kimchi quality. This study aimed to investigate the effect of different fermentation temperatures (4, 10, and 20 °C) and packaging conditions (open or closed) on WCFY diversity, and the changes of metabolite by the difference of WCFY diversity. Community analysis using high-throughput DNA sequencing revealed that Kazachstania servazzii and K. barnettii were most prevalent in kimchi fermented under closed packaging condition at 4, 10, and 20 °C. In open packaging condition, four species of Candida sake, K. servazzii, K. barnettii, and Tausonia pullulans were the predominant yeast species at 4 °C, and four species of C. sake, K. servazzii, K. barnettii, and Debaryomyces hancenii were predominantly detected at 10 °C. The diversity of the WCFY community was higher under the open rather than the closed packaging condition. However, at all fermentation temperatures, non-volatile metabolite production by the different WCFY communities did not significantly differ between open and closed packaging conditions, whereas glycerol levels in kimchi samples harboring WCFY increased relative to the control (0 day). These results indicate that fermentation temperature and air exposure can alter WCFY diversity on kimchi surface, however, non-volatile metabolite profiles in kimchi soup are not significantly affected by the difference of WCFY diversity caused by packaging conditions. This study furthers the current understanding of the growth of undesirable WCFY in kimchi.Theoretical and empirical models about the coexistence of formation and decomposition of hydroperoxide during lipid oxidation under Rancimat conditions were established, verified and compared in this research. Theoretical models were proposed based on the simplified three steps of free radical chain reactions by solving kinetic differential equations, giving both formation and decomposition equations. Empirical models were modified from former phenomenological equations by eliminating the parameter of inflection time. Two groups of equations were both well verified by 93 sets of experimental data with adj-R2 of 0.9585-0.9967 and 0.9431-0.9977. Theoretical models were only suitable for the accumulation period of hydroperoxide, while empirical models were able to describe the whole process but give the inappropriate formation and decomposition equations. The further applications of models in quantitatively defining oxidation stages (lag, exponential and stationary phases) and re-evaluating the total oxidation value (TOTOX' = 2.27*PV + AnV) were also introduced.Proton nuclear magnetic resonance (1H NMR)- and ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS)-based analytical tools are frequently used in metabolomics studies. These complementary metabolomics platforms were applied to identify and quantify the metabolites in Phyllanthus acidus extracted with different ethanol concentrations. In total, 38 metabolites were tentatively identified by 1H NMR and 39 via UHPLC-MS, including 30 compounds are reported for the first time from this plant. The partial least square analysis (PLS) revealed the metabolites that contributed to α-glucosidase and nitric oxide (NO) inhibitory activities, including kaempferol, quercetin, myricetin, phyllanthusol A, phyllanthusol B, chlorogenic, catechin, cinnamic coumaric, caffeic, quinic, citric, ellagic and malic acids. This study shows the significance of combining 1H NMR- and UHPLC-MS-based metabolomics as the best strategies in identifying metabolites in P. acidus extracts and establishing an extract with potent antioxidant, anti-diabetic, and anti-inflammatory properties.
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