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https://www.selleckchem.com/products/n6f11.html The structure of the polysaccharide O-chain of the lipopolysaccharide isolated from the sequenced strain Chromobacterium violaceum ATCC 12472 (NCTC 9757) was investigated by chemical and NMR analyses, and concluded to be -4-α-Leg5Ac7Ala-4-β-d-ManNAlaA3OAc-3-α-d-GlcNAc-where Leg5Ac7Ala indicates 5-acetamido-7-alanylamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulopyranosonic acid and ManNAlaA3OAc 3-O-acetyl-2-alanylamido-2-deoxymannopyranuronic acid. The structure of the core with one repeating unit of the polysaccharide attached was also analyzed, and it was found that the O-chain polysaccharide is linked to the core via β-GlcpNAc, as opposite to α-GlcpNAc inside the O-chain. A colorimetric microassay for the quantitative determination of galactose in the blood was taken and updated. This method helps in diagnosis and follow-up of several inherited metabolic diseases connected to galactose metabolism deficiency such as galactosemia, glycogenosis, glycosylation, tyrosinemia and citrin deficiency. Galactose assay in the blood presents difficulties due to interference with glucose. In this study, we update a method to get around these difficulties. This procedure was based on the incubation of whole blood with orcinol in a strongly acidic solution to form a galactose and glucose complexes able to absorb at two different wavelengths. The standard curve analysis for the individual solutions of these two sugars showed a wide range of linearity from 0 to 200 mg / l. Under optimal experimental conditions, the stirring time of the orcinol is 3 minutes, the heating time of the reaction is 20 minutes at 56 ° C, and the duration of the incubation in the dark is 40 minutes. The analysis is carried out on fresh blood. The maximum absorbance of galactose and glucose is respectively 569 nm and 421 nm. An adapted diagnosis algorithm was developed based on our results. this method could help in screening and identifying patients wi
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