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https://www.selleckchem.com/products/BIRB-796-(Doramapimod).html NA.Sclerotium rolfsii is a fungi pathogen of southern blight with broad host range. The QoI fungicide pyraclostrobin was officially approved for controlling many diseases in 2015. In this study, baseline sensitivity of S.rolfsii to pyraclostrobin was established by measuring the 50% effective concentration (EC50) values of 155 isolates of S.rolfsii. The EC50 ranged from 0.0291 to 1.0871 ug/ml with the mean EC50 values of 0.4469 ± 0.2490 ug/ml (mean ± SD). As preventive fungicide in vitro and field experiment, pyraclostrobin preventive efficacy reached 90% and 80%, respectively, which were much higher than that of control agent carbendazim. Curative efficacy of pyraclostrobin was significantly lower than its preventive efficacy. Pyraclostrobin at 0.1, 0.5, and 2 μg/mL significantly reduced the number of sclerotia produced on potato dextrose agar (PDA) medium, but had no significant influence on their total weight. Pyraclostrobin had no significant influence on mycelial cell membrane permeability, but it significantly reduced oxalate secretion and protein synthesis of S. rolfsii. Our findings are of great significance for resistance monitoring of S. rolfsii and also provide new insight into action mechanism of pyraclostrobin against S. rolfsii.Cyprus is the southernmost island country of Europe located in the Mediterranean and despite its limited area, potato production is considered an integral source of the national agricultural revenue. During 2010-2012, a late blight-epidemic period for the country, the population structure of Phytophthora infestans was analyzed via a sample of 539 isolates collected from all the main potato-cultivating regions of Cyprus. We determined mating type, mefenoxam sensitivity, and genetic polymorphism at 12 simple sequence repeat (SSRs) loci. Although both mating types were detected in the country, a gradual -but dynamic- shift towards A2 dominance was manifested over time

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