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https://www.selleckchem.com/products/ml355.html A quick, easy, cheap, effective, rugged and safe (QuEChERS) method was optimized for the extraction of non-steroidal anti-inflammatory drugs (NSAIDs) diclofenac and ibuprofen from sewage sludge. Dispersive-solid phase extraction (d-SPE) was employed for sample clean-up. Instrumental analysis was performed by high-performance liquid chromatography. Ecological risk was assessed for four trophic levels fish, daphnia, algae and bacteria. The method limits of quantification for diclofenac and ibuprofen were 0.43 µg g- 1 and 0.45 µg g- 1, respectively. Correlation coefficients were above 0.999. Extraction recoveries ranged from 70 to 118 % and satisfactory inter-day reproducibility (% RSD) of less then  18 % was obtained. Diclofenac and ibuprofen were measured up to 1.02 µg g- 1 and 6.6 µg g- 1, respectively in sewage sludge from three Nigerian wastewater treatment plants (WWTPs). Ibuprofen posed high risk to fish, daphnia, algae and bacteria. This work presents the first report on the ecological risk assessment of diclofenac and ibuprofen in sewage sludge from Nigerian WWTPs. To investigate the prognostic role of the preoperative systemic immune-inflammation index (SII) in patients with upper tract urothelial carcinoma (UTUC) treated with radical nephroureterectomy (RNU). We retrospectively analyzed our multi-institutional database to identify 2492 patients. SII was calculated as platelet count × neutrophil/lymphocyte count and evaluated at a cutoff of 485. Logistic regression analyses were performed to investigate the association of SII with muscle-invasive and non-organ-confined (NOC) disease. Cox regression analyses were performed to investigate the association of SII with recurrence-free, cancer-specific, and overall survival (RFS/CSS/OS). Overall, 986 (41.6%) patients had an SII > 485. On univariable logistic regression analyses, SII > 485 was associated with a higher risk of muscle-invasive (P = 0.004) and NOC (P = 0.

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