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https://www.selleckchem.com/products/sri-011381.html Novel cytogenetic tools are increasingly based on genome sequencing for detecting chromosomal abnormalities. Different sequence-based techniques optimized for diagnosis of structural variants can be useful for narrowing down the localization of breakpoints of chromosomal abnormalities, but do not offer nucleotide resolution of breakpoints for proper interpretation of gene disruption. This protocol presents the characterization of structural variants at nucleotide resolution using Sanger sequencing after low-pass large-insert genome sequencing or other long-molecule methods. © 2020 Wiley Periodicals LLC. Basic Protocol 1 Primer design for junction amplification at translocations and inversions Basic Protocol 2 Amplification of derivative chromosomes using a long-range polymerase Alternate Protocol Amplification of derivative chromosomes using a hot-start polymerase Basic Protocol 3 Preparation of DNA for Sanger sequencing Basic Protocol 4 Interpretation and reporting of breakpoints based on Sanger sequencing.Antimicrobial peptides (AMPs) are expected to be good candidate molecules for novel antimicrobial therapies. Most AMPs exert their antimicrobial activity through disruption of microbial membranes due to their amphipathic properties. Recently, the helical peptide 'Stripe' was reported by our group, a rationally designed amphipathic AMP focused on distribution of natural cationic and hydrophobic amino acid residues. In this study, a set of Stripe-based AMP foldamers was designed, synthesized and investigated that contain α,α-disubstituted amino acids or side-chain stapling to stabilize their helical structures. Our results showed that a peptide containing 2-aminoisobutyric acid (Aib) residues exhibited potent antimicrobial activity against both Gram-positive S.aureus (MIC value 3.125 μM) and Gram-negative bacteria (including a multidrug-resistant strain, MDRP, MIC value 1.56 μM), without significant hemolytic act

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