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https://www.selleckchem.com/products/n6022.html Duplex-specific nuclease signal amplification (DSNSA) is a promising microRNA (miRNA) quantification strategy. However, existing DSNSA based miRNA detection methods suffer from costly chemical consumptions and require laborious multi-step sample pretreatment that are prone to sample loss and contamination, including total RNA extraction and enrichment. To address these problems, herein we devised a pneumatically automated microfluidic reactor device that integrates both analyte extraction/enrichment and DSNSA-mediated miRNA detection in one streamlined analysis workflow. Two flow circulation strategies were investigated to determine the effects of flow conditions on the kinetics of on-chip DSNSA reaction in a bead-packed microreactor. With the optimized workflow, we demonstrated rapid, robust on-chip detection of miR-21 with a limit-of-detection of 35 amol, while greatly reducing the consumption of DSN enzyme to 0.1 U per assay. Therefore, this microfluidic system provides a useful tool for many applications, including clinical diagnosis.Mycotoxins contamination in agricultural products poses a serious threat to human and animal health, so rapid and sensitive nanosensors for simultaneous determination of multiple mycotoxins in food samples are highly desirable for food safety monitoring. Herein, we report the fabrication of functional dual-colored persistent luminescence nanoparticles (PLNPs) in conjunction with Fe3O4 magnetic nanoparticles as a nanosensor for the simultaneous biosensing of aflatoxin B1 (AFB1) and zearalenone (ZEN) in food samples. Two types of PLNPs with a single excitation wavelength, Zn2GeO4Mn2+ and Zn1.25Ga1.5Ge0.25O4Cr3+,Yb3+,Er3+, are employed as the signal units, and aptamers with high affinity and specificity to the corresponding mycotoxins are used as the recognition units. The nanosensor was fabricated by hybridizing the aptamer modified PLNPs with the complementary DNA modified Fe3O4. The de

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