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https://www.selleckchem.com/products/cb-839.html This work reports on the method optimization and application for quantitative analysis of non-steroidal anti-inflammatory drugs and anti-epileptic drug in soil and sediment samples. The analytes were extracted by ultrasonic extraction followed by solid phase extraction and quantified using liquid chromatographic coupled with photodiode array. The sensitivity of the method was determined based on the limit of detection and the limit of quantification which ranged between (0.010-0.027 µg/kg) and (0.025-0.049 µg/kg), respectively. The %recoveries of the method ranged between 74% and 112%. The concentrations obtained in real samples ranged from 0.055 to 0.426 µg/kg in sediment and 0.044-0.567 µg/kg in soil samples. The highest concentration was found for diclofenac in soil samples.Orthopedic and trauma surgery are currently confronted with significant changes in their interventional and therapeutic strategies, especially in knee surgery. Minimally invasive and reconstructive techniques lead not only to modifications of the repertoire of interventional methods but also of the indications and questions for histopathological diagnostics. The classical problems in knee surgery remain important issues, which are traumatic, degenerative, and infectious lesions. In addition, questions regarding regeneration and integration of cell-material constructs will become more and more important in the future. Furthermore, questions regarding the regenerative potential of an implantation site for such constructs and the morphological quality of harvested tissue for the in vitro cell expansion of autologous cells are becoming increasingly important. The autologous chondrocyte transplantation is a good example of the relevance of the histopathological re-evaluation of the regenerated tissue for a better understanding of the pathophysiology of reconstructive therapies. It becomes clear that for specific aims based on reconstructive therapies

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