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A versatile approach to entrap relatively small enzymes in hydrogels allows their diverse biotechnological applications. In the present work, bovine carbonic anhydrase (BCA) was efficiently entrapped in calcium alginate beads with the help of liposomes. A mixture of sodium alginate (3 wt %) and carbonic anhydrase-liposome conjugates (BCALs) was dripped into a Tris-HCl buffer solution (pH = 7.5) containing 0.4 M CaCl2 to induce the gelation and curing of the dispersed alginate-rich droplets. The entrapment efficiency of BCALs, which was defined as the amount of catalysts entrapped in alginate beads relative to that initially charged, was 98.7 ± 0.2% as determined through quantifying BCALs in the filtrate being separated from the beads. When free BCA was employed, on the other hand, a significantly lower entrapment efficiency of 27.2 ± 4.1% was obtained because free BCA could pass through alginate matrices. Because the volume of a cured alginate bead (10 μL) entrapped with BCALs was about 2.5 times smaller than that of an original droplet, BCALs were densely present in the beads to give the concentrations of lipids and BCA of 4.6-8.3 mM and 1.1-1.8 mg/mL, respectively. Alginate beads entrapped with BCALs were used to catalyze the hydrolysis of 1.0 mM p-nitrophenyl acetate (p-NA) at pH = 7.5 using the wells of a microplate or 10 mL glass beakers as batch reactors. Furthermore, the beads were confined in a column for continuous-flow hydrolysis of 1.0 mM p-NA for 1 h at a mean residence time of 8.5 or 4.3 min. The results obtained demonstrate that the conjugation of BCA to liposomes gave an opportunity to achieve efficient and stable entrapment of BCA in alginate hydrogels for applying to catalytic reactions in bioreactors.This work reports a simple water-phase microwave method for the synthesis of water-soluble red emission Ag2S quantum dots at low temperatures without the need for an anaerobic process. It is worth noting that the prepared water-soluble Ag2S quantum dots enjoy positive water dispersion stability. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT) results showed that the prepared Ag2S quantum dots had promising biocompatibility and low cytotoxicity. In addition, we further applied the low-toxicity near-infrared Ag2S quantum dots for cell imaging, demonstrating a promising biological probe for cell imaging.A zero valent iron-loaded nano-bentonite intercalated carboxymethyl chitosan (nZVI@nBent-CMC) composite was fabricated and characterized by FT-IR, TEM, TEM-EDX, XRD, BET surface area, and zeta potential measurements. The as-fabricated nZVI@nBent-CMC composite exhibited excellent removal efficiency for both anionic Congo red (CR) dye and cationic crystal violet (CV) dye. The maximum uptake capacities of CR and CV onto the nZVI@nBent-CMC composite were found to be 884.95 and 505.05 mg/g, respectively. The adsorption process of both dyes well fitted with the Langmuir isotherm model and pseudo-second order kinetic model. Thermodynamic data clarified that the adsorptions of both CR and CV onto the nZVI@nBent-CMC composite are spontaneous processes. Moreover, the adsorption of CR onto the nZVI@nBent-CMC composite was found to be an exothermic process while that of CV is an endothermic process. The nZVI@nBent-CMC composite also exhibited excellent reusability for both studied dyes without noticeable loss in the removal efficiency, suggesting its validity to remove both anionic and cationic dyes from wastewater.The present paper analyzes the entropy generation of a tabular thermal cracking reactor with propane feed, as well as the molecular mechanism of the reaction. The reaction model is obtained and the flow, reaction, momentum, and energy are determined with knowledge of the outer wall temperature. Then, the equations of entropy generation in the tabular reactor are solved and the rate of entropy generated by three sources along the reactor is determined. https://www.selleckchem.com/products/pf429242.html After that, the temperature of the tube or reactor is changed using the reference results, and the results and procedures are replicated. The results obtained for two states show that the three factors are in the order of chemical reactions, heat transfer, and pressure loss in terms of their contribution to entropy generation. The decrease in input feed temperature does not influence the weight percent of the products considerably, but when the wall temperature is reduced, as is the case with the optimal wall temperature state, the conversion percent in the reactor is reduced. The entropy generated by heat transfer in reactors with lower feed temperature is much higher at the beginning of the reaction. These variations do not affect the pressure-induced entropy considerably. The variation of input feed temperature does not affect the entropy generated by the chemical reactions, but the entropy produced in the reactor with optimal wall temperature is the lowest. At the inlet of the reactor, the entropy is higher when the wall has optimal temperature, which can be attributed to its higher rate of reactions than the other two states of input feed temperature.A boron-doped diamond (BDD) has been widely used as an outstanding electrode for constructing high-performance electrochemical biosensors. In this paper, we fabricated a novel electrode combined of nanometer-sized graphite-BDD film (NG-BDD) by chemical vapor deposition. The nanometer-sized graphite (NG) is formed on the (111) facet of BDD via converting an sp3 diamond structure to an sp2 graphitic phase at high temperature in boron-rich ambient. The electrode was characterized by means of scanning electron microscopy, Raman spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. This NG-BDD was performed as an electrode of electrochemical biosensor to detect trace acetaminophen (APAP) accurately. Cyclic voltammetry and differential normal pulse voltammetry are used to investigate the overall performance of the electrochemical device. The sensor has a linear electrochemical response to APAP in the concentration range of 0.02-50 μM, and the detection limit is estimated to be as low as 5 nM. The research has resulted in a solution of constructing a reusable NG-BDD sensor to detect APAP with stability and show potential in extensive application.
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