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https://www.selleckchem.com/products/FK-506-(Tacrolimus).html The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples. Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated. DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode. The limit of detection (LOD) is estimated to approximately 50 pg/spot on bone tissue. The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle. The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios. The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations. Pain among adolescents is prevalent that may negatively affect adolescents' general well-being of which sleep is an important domain. This study aims to explore the associations between weekly pain and medicine use for relevant pain and insufficient sleep duration among 11-, 13- and 15-year-old adolescents in Latvia by assessing the moderation effect of gender and age. Data from the Health Behaviour in School-aged Children Study on 2017/2018 of Latvia (n = 4412; 49.6% boys) were used. Logistic regression was applied to analyse the odds of insufficient sleep (< 7h) on schooldays and weekends in association with weekly headache, stomach ache or backache and corresponding medicine use when testing the interaction effect of adolescents' gender and age. The experience of weekly pain with or without medicine use significantly increased the odds of insufficient sleep compared to adolescents with pain less than weekly, whilecontrolling for gender and age. The interaction effect of gender and age on the studied associations was not significant. We

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