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https://www.selleckchem.com/ We previously constructed a heterologous production system for ergothioneine (ERG) in Escherichia coli using five ERG biosynthesis genes (egtABCDE) from Mycobacterium smegmatis. However, significant amounts of hercynine (HER), an intermediate of ERG, as ERG were accumulated, suggesting that the reaction of EgtB catalyzing the attachment of γ-glutamylcysteine (γGC) to HER to yield hercynyl-γ-glutamylcysteine sulfoxide was a bottleneck. In this study, we searched for other EgtBs and found many egtB orthologs in diverse microorganisms. Among these, Methylobacterium strains possessed EgtBs that catalyze the direct conversion of HER into hercynylcysteine sulfoxide with l-cysteine (l-Cys) as a sulfur donor, in a manner similar to those of acidobacterial CthEgtB and fungal Egt1. An in vitro study with recombinant EgtBs from Methylobacterium brachiatum and Methylobacterium pseudosasicola clearly showed that both enzymes accepted l-Cys but not γGC. We reconstituted the ERG production system in E. coli with egtB from M. pseudosasicola; ERG productivity reached 657 mg L-1.Due to the high energy efficiency and a wide range of potential applications in daily life, bistable electrochromic devices (BECDs) have gained extensive attention in recent years. However, poor bistability and slow response rate are the main barriers, which restrict the development of BECDs. Herein, a transparent multidimensional electrode with indium tin oxide (ITO) nanofibers and gold nanoparticles was fabricated to improve both the bistability and responsiveness of devices. Its criss-crossed ITO nanofibers with high length-to-diameter ratios to reduce the distance of heterogeneous electron transfer and its gold nanoparticles to form a stable Au-S bond to avoid self-erasing of electrochromic materials have been demonstrated and discussed systematically. This device based on the transparent multidimensional working electrode had a shorter response time (1/5) and a longer bistabi

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