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https://www.selleckchem.com/products/ldk378.html On level, the metabolic cost (C) of backward running is higher than forward running probably due to a lower elastic energy recoil. On positive gradient, the ability to store and release elastic energy is impaired in forward running. We studied running on level and on gradient to test the hypothesis that the higher metabolic cost and lower efficiency in backward than forward running was due to the impairment in the elastic energy utilisation. Eight subjects ran forward and backward on a treadmill on level and on gradient (from 0 to + 25%, with 5% step). The mechanical work, computed from kinematic data, C and efficiency (the ratio between total mechanical work and C) were calculated in each condition. Backward running C was higher than forward running at each condition (on average + 35%) and increased linearly with gradient. Total mechanical work was higher in forward running only at the steepest gradients, thus efficiency was lower in backward running at each gradient. Efficiency decreased by increasig the recently introduced 'equivalent slope' concept for running acceleration, to obtain the predictive equation of metabolic power during level backward running acceleration.This investigation sought to determine whether post-exercise cold water immersion and low glycogen availability, separately and in combination, would preferentially activate either the Exon 1a or Exon 1b Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) promoter. Through a reanalysis of sample design, we identified that the systemic cold-induced augmentation of total PGC-1α gene expression observed previously (Allan et al. in J Appl Physiol 123(2)451-459, 2017) was largely a result of increased expression from the alternative promoter (Exon 1b), rather than canonical promoter (Exon 1a). Low glycogen availability in combination with local cooling of the muscle (Allan et al. in Physiol Rep 7(11)e14082, 2019) demonstrated tha

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