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https://www.selleckchem.com/products/jnj-64264681.html 5 mg/L), which is beneficial to decrease energy requirements. Biochemical methane potential (BMP) tests showed better productivity for the anaerobic sludge than the aerobic sludge, with an optimal BMP of 296±2 mL CH4/gVSS. FISH analysis at SRT = 4d revealed a high abundance of Accumulibacter (33±13%) and lower proportion of GAO Competibacter (3.0±0.3%), Defluviicoccus I (0.6±0.1%) and Defluviicoccus II (4.3±1.1%).The entering of the widespread polyethylene terephthalate (PET) microplastics into biological wastewater treatment system results in their retention in sewage sludge, which inevitably enters the sludge treatment system. However, all previous studies regarding the impact of microplastics on sludge treatment system were conducted by directly adding microplastics to system and focusing on anaerobic sludge digestion, although PET microplastics commonly enter into the biological wastewater treatment system first before sludge being subsequently treated. The potential impact of the microplastics on waste activated sludge (WAS) aerobic digestion is also completely missing. Therefore, herein the influences of PET microplastics with different entry paths on WAS aerobic digestion as well as the key mechanisms involved was firstly explored. Experimental results demonstrated that compared to the control test, the entering of PET microplastics to biological wastewater treatment system inhibited WAS aerobic digestion by 10.9 ± 0.1% through the decreased hydrolysis, although WAS solubilization during aerobic digestion was improved due to the change of generated WAS characteristics. In contrast, when PET microplastics was directly added to the sludge aerobic digester, there was little impact on solubilization, while the hydrolysis were inhibited seriously, thereby suppressing WAS aerobic digestion more severely by 28.9 ± 0.1%. Further investigation revealed that PET microplastics reduced the populations of key bacteria

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