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https://www.selleckchem.com/products/frax486.html The concentration of primary chlorine-substituted products was about 4 times that of the dimer products, demonstrating that electrophilic substitution reaction was predominant during chlorination of 2,4-DBP. Density functional theory (DFT) based calculations revealed that HOCl is the dominant active oxidizing species for elimination of 2,4-DBP and coupling reaction occurs more easily at para and ortho position of hydroxyl group in the phenolic moiety. These findings could provide some new insights into the environmental fate of bromophenols during chlorine disinfection of water and wastewaters.White-rot fungi are well known bioremediation agents capable of removing recalcitrant xenobiotics. However, the molecular mechanism involved in this process is not well understood. The aim of the present study was to compare the proteomic profiles of Pleurotus pulmonarius LBM 105 in presence and absence of a mixture of polychlorinated biphenyls. Cultures of the fungus were spiked with a mixture of Aroclors and cultivated for 28 days. This strain achieved a peak of PCBs-removal of 65.50 ± 8.09% after 21 days. The ecotoxicological assays showed a toxicity reduction of 46.47%. Based on these findings, a proteomic study was carried out and it was proven that the oxidative metabolism was highly affected. Two proteins that have a function at the transcriptional level and related to the oxidative metabolism, the glyceraldehyde-3-phosphate dehydrogenase and the basal transcription factor 3, presented an increase in their quantity in PCBs presence. Several oxidases and reductases were highly induced, presenting the short chain reductases, aldo/keto reductases, laccases and versatile peroxidases as the enzymes with the most notorious changes. These results indicate a complex response of the fungal metabolism towards these pollutants, which includes a transcriptional response to the oxidative stress and a modification of the intra- and ex

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