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https://www.selleckchem.com/products/5-chloro-2-deoxyuridine.html 05). Network analysis showed that bio-stimulation substantially increased the dominant bacterial community's proportion, especially Proteobacteria. The functional gene results illustrated that bio-stimulation facilitated total relative abundance of degradation genes, phosphorus, carbon, nitrogen, sulfur metabolic genes, and iron transporting genes (p less then 0.05). In metabolic pathways, functional genes related to methanogenesis and ammonia generation were markedly upregulated, indicating that bio-stimulation promoted the transformation of metabolic genes, such as carbon and nitrogen. This research is conducive to exploring the microbiological response mechanisms of bio-stimulation in indigenous flora, which may provide technical support for assessing the microbial ecological remediation outcomes of bio-stimulation in OCP contaminated sites.The main goal of this work is to evaluate the usage of ozone (O3) as a pre-treatment or simultaneously combined with UVC/H2O2 process for the polishing stage treatment of real bio-treated slaughterhouse wastewater. Two different treatment strategies were tested i) pre-ozonation of the wastewater followed by an UVC/H2O2 process (two-step treatment); ii) simultaneous application of O3/UVC/H2O2 combined process (one-step treatment). For the two-step strategy, the pre-treatment with 30 mg O3/min for 10 min reduces significantly total suspended solids (TSS), turbidity and colour, reducing light filtering effects and increasing the efficiency of the following UVC/H2O2 process. In turn, the one-step treatment strategy (O3/UVC/H2O2) allows a more efficient use of injected O3 by reducing the amount of O3 required (from 273 to 189 mg O3/Leffluent) to achieve similar mineralization levels. The real bio-treated slaughterhouse wastewater treated by O3/UVC/H2O2 process achieved final colour values of 20 Pt/Co, TSS of 35 mg/L and COD of 61 mg O2/L, allowing its direct discharg

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