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https://www.selleckchem.com/products/cay10444.html Microbial community-based polyhydroxyalkanoate (PHA) production has been demonstrated repeatedly at pilot scale. Ammonium, normally present in waste streams, might be oxidized by nitrifying bacteria resulting in additional aeration energy demand. The use of low dissolved oxygen (DO) concentrations allowed to reduce nitrifying rates by up to 70% compared to non-oxygen limiting conditions. At lower DO concentrations nitrate was used as alternative electron acceptor for PHA production and therefore, a constant PHA production rate could only be maintained if nitrate was sufficiently available. An optimum DO concentration (0.9 mgO2/L) was found for which nitrification was mitigated but also exploited to supply requisite heterotrophic nitrate requirements that maintained maximum PHA production rates. PHA accumulations with such DO control was estimated to reduce oxygen demand by about 18%. This work contributes to establish fundamental insight towards viable industrial practice with the control and exploitation of nitrifying bacteria in microbial community-based PHA production.A process combining hydrothermal treatment (HT), pyrolysis, and anaerobic digestion can efficiently treat antibiotic fermentation residues (AFR). The process characteristics and antibiotic resistance genes (ARGs) removal efficiencies of each unit have been investigated. HT of 180 °C improved the biodegradability and dewaterability of the AFR. Pyrolysis of 500 °C and upflow anaerobic sludge blanket (UASB) of 6.5 ± 0.5 kg COD•(m3•d)-1 recovered the organic matter in filter cake and filtrate of AFR. The biogas and pyrolysis gas can compensate the energy this system needs. HT of 180 °C could reduce 16S rRNA, ARGs, and mobile genetic elements (MGEs) by 2.3 to 7.4 logs. UASB increased the copy numbers of ARGs and MGEs, but the relative abundances of ARGs normalized against 16S rRNA were significantly declined. The ARGs and MGEs were enriched in suspended

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