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https://www.selleckchem.com/products/jq1.html OBJECTIVE To examine the construct validity, reliability and responsiveness of the Otology Questionnaire Amsterdam (OQUA). DESIGN Multicentre, longitudinal study in 2 separate cohorts of patients visiting an ENT surgeon via an online survey programme. SETTING Tertiary ENT clinics. PARTICIPANTS Cohort 1 consisted of patients at their first visit at an ENT outpatient clinic with an ear complaint. Cohort 2 consisted of patients who underwent surgery, with a 3-month follow-up post-surgery. MAIN OUTCOME MEASURES Construct validity Hypothesis testing, internal consistency and inter-item correlation. Reliability Test-retest reliability. The construct approach was used for assessing responsiveness. Hypotheses were formulated based on the association between the OQUA and Glasgow Health Status Inventory (GHSI) or Global Rating Scale (GRS). RESULTS Construct validity The correlation between the individual items in the impact domain ranged from 0.424 to 0.737. Confirmatory factor analysis showed a good fit. As expected, ohn Wiley & Sons Ltd.To enhance the angiogenic capacity of tissue-engineered peripheral nerves, we have constructed revascularized tissue-engineered nerves based on a vascular endothelial growth factor (VEGF)-heparin sustained release system. However, the effects of the repair of large peripheral nerve defects are not known. In this study, we used the above revascularized tissue-engineered nerve to repair large nerve defects in rats. The repair effects were observed through general observation, functional evaluation of nerve regeneration, ultrasound examination, neural electrophysiology, wet weight ratio of bilateral gastrocnemius muscle, histological evaluation, and quantitative real-time polymerase chain reaction (PCR) analysis. The results showed that the tissue-engineered peripheral nerve based on a VEGF-heparin sustained release system can achieve early vascularization and restore blood supply in the nerve graft

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