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The consumption of fresh rabbit meat has become more popular among consumers in recent years, but they are easily perishable. In this study antioxidant and antimicrobial effects of mulberry (Morus nigra) leaves extract (MLE) and olive (Olea europaea) leaves extract (OLE) dip treatments at 2% w/v on the quality attributes and shelf-life of fresh rabbit meat during chilling storage under aerobic conditions were investigated. Rabbit meat samples were refrigerated at 4±1°C to be periodically examined for their sensory quality, physicochemical parameters and bacteriological status. Results indicated that as the time of cold storage progressed, the overall mean scores of physicochemical and microbiological parameters were increased, while sensory scores were decreased (p<0.05) irrespective of treatment. Both natural extracts (MLE/OLE) significantly (p<0.05) delayed oxidative quality changes, protein deterioration and proliferation of bacteria noticed during the chilling study. Olive leaves extract (OLE; 2%) was more significant (p<0.05) positively affect than mulberry leaves extract (MLE; 2%) in maintaining chemical indices, lipid stability, consumer acceptance, microbial load and can prolonged the expiry of treated rabbit meat by 4 days as compared to control one. Hence, the potential of olive leaves extract to preserve rabbit meat during cold storage has been demonstrated. Olive leaves extract (OLE; 2%) was more significant (p less then 0.05) positively affect than mulberry leaves extract (MLE; 2%) in maintaining chemical indices, lipid stability, consumer acceptance, microbial load and can prolonged the expiry of treated rabbit meat by 4 days as compared to control one. Hence, the potential of olive leaves extract to preserve rabbit meat during cold storage has been demonstrated. Shallot is a vegetable crop with high economic value, but its productivity is still relatively low due to various limitations. One of the most hampering factors is moler disease and purple blotch disease caused by Fusarium sp. and Alternaria porri, respectively. Numerous efforts have been made to control these diseases either using chemical fungicides or through improvement of resistant cultivar. This study aimed to determine moler and purple disease suppression and improvement of plant growth by Bacillus as Plant Growth Promoting Rhizobacteria (PGPR) on shallot. Molecular identification of Bacillus was performed by partial gyrB gene sequencing using universal gyrB-F/gyrB-R primers. Field observation and experiments were performed using completely randomized factorial block design single factor with 3 blocks for replication. The partial gyrB gene sequences showed high similarity between Bacillus isolate B-27 and Bacillus velezensis. The application of Bacillus isolate B-27 to shallots was shown to reduce the intensity of moler and purple blotch diseases by 67%. On top of that, Bacillus isolate B-27 increased the plant height up to 27.12 cm, the number of leaves up to 23 blades, tillers up to 8 bulbs and the tuber weight during harvest time up to 33.64 kg. Molecular identification based on partial gyrB gene sequence analysis suggested that Bacillus isolate B-27 has close relationship with Bacillus velezensis. Besides, the application of Bacillus isolate B-27 on shallot could reduce the disease intensity and increase height, number of tillers and plant yield significantly. Molecular identification based on partial gyrB gene sequence analysis suggested that Bacillus isolate B-27 has close relationship with Bacillus velezensis. Besides, the application of Bacillus isolate B-27 on shallot could reduce the disease intensity and increase height, number of tillers and plant yield significantly. The malathion is one of the most important organophosphorus pesticides used in Iraq. The present study was designed to investigate the short-time effects of the malathion on the biochemical parameters of AST, ALT, ALP, urea, creatinine, total cholesterol, triglycerides and total protein as well as histological changes of the liver and kidneys of female laboratory mice for an interval of 6 days. The animals were divided into 3 groups, each group included 8 mice. They were injected with the pesticide in the intraperitoneal region. The 1st group (the control group) was injected with 0.1 mL of distilled water, the 2nd group (the low dose group) was injected with 0.1 mL of the pesticide solution at 3 mg/body weight while the 3rd group (the high dose group) was injected with 0.1 mL of the pesticide solution at a concentration of 6 mg/body weight. The biochemical tests of the liver and kidney showed significant elevation in serum AST, urea, creatinine and cholesterol concentrations in mice compared to control group (p<0.05). https://www.selleckchem.com/products/gbd-9.html In addition, the results showed a significant decrease in the ALP, triglycerides and the total protein in serum of the treated mice. Also, the results of histological sections of the liver and kidneys included congestion, necrosis, degeneration of cytoplasm, blood congestion, apoptosis, bleeding and sloughing of epithelial cells to the renal tubular lumen. Finally, the results indicated that malathion pesticide has the ability to induce hepatic and renal toxicity in mice within 6 days. Finally, the results indicated that malathion pesticide has the ability to induce hepatic and renal toxicity in mice within 6 days. The retina is critical for vision, and several diseases may alter its biomechanical properties. However, assessing the biomechanical properties of the retina nondestructively is a challenge due to its fragile nature and location within the eye globe. Advancements in Brillouin spectroscopy have provided the means for nondestructive investigations of retina biomechanical properties. We assessed the biomechanical properties of mouse retinas using Brillouin microscopy noninvasively and showed the potential of Brillouin microscopy to differentiate the type and layers of retinas based on stiffness. We used Brillouin microscopy to quantify stiffness of fresh and paraformaldehyde (PFA)-fixed retinas. As further proof-of-concept, we demonstrated a change in the stiffness of a retina with N-methyl-D-aspartate (NMDA)-induced damage, compared to an undamaged sample. We found that the retina layers with higher cell body density had higher Brillouin modulus compared to less cell-dense layers. We have also demonstrated that PFA-fixed retina samples were stiffer compared with fresh samples.
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