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https://www.selleckchem.com/products/e1210.html The developed method could process up to 50 ml of lettuce cocktail blended from 5g samples and purposefully inoculated with E. coli O157H7. Overall, the enrichment method developed required only 40 min of sample processing time. After enrichment, as low as 100 CFU/ml of E. coli O157H7 could be detected by a simple colorimetric procedure due to the enhancement from the proposed checkpoint-style enrichment in the presence of ∼3000 CFU/ml of non-target bacteria. A linear response was obtained from blank to 100000 CFU/ml of E. coli O157H7 in blended lettuce samples. The conceptualized approach demonstrates a promising means to improve the detection of target bacteria with a high degree of sensitivity and specificity and could be used in low resourse settings.In this work, a surface-enhanced Raman scattering (SERS) sensing strategy was proposed for the analysis of lead ion (Pb2+) with high sensitivity and specificity based on the high specificity of the catalytic nucleic acids (DNAzymes) to Pb2+ and the catalytic hairpin assembly (CHA) amplification. First, the Pb2+-DNAzyme initiated the reaction by target Pb2+ and released a linear DNA (rS1). Second, the hairpin DNA 1 (H1) was immobilized on the SERS substrate surface via Ag-S bond. Then, the rS1 could cyclically trigger the allosteric effects of CHA amplification and the H1 was opened and then the R6G-labeled hairpin probe 2 (H2) hybridized with H1 to form H1-H2 double-stranded and the released rS1 could initiate the next cycle of CHA reaction. This process made the Raman tag of R6G close to the surface of SERS substrate, and the intensity of SERS signal from R6G labels increase with the increase of concentration of target Pb2+. Benefiting from outstanding performances of the Pb2+-specific DNAzymes and enzyme-free CHA amplification system, this biosensor exhibits good sensitivity for Pb2+ with a limit of detection of 0.42 pM. More importantly, this developed detection pla

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