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https://www.selleckchem.com/products/thal-sns-032.html 05). The maximal isometric force/cross-sectional area generated by isolated membrane-permeabilised muscle fibres was determined. Results None of the 426 variants identified in 16 candidate genes were associated with MFM including 26 missense variants. Breed-specific differences existed in allele frequencies. Candidate gene differential expression and muscle fibre-specific force did not differ between MFM WB (143.1 ± 34.7 kPa) and non-MFM WB (140.2 ± 43.7 kPa) (P = .8). Main limitations RNA-seq-only assays transcripts expressed in skeletal muscle. Other possible candidate genes were not evaluated. Conclusions Evidence for association of variants with a disease is essential because coding sequence variants are common in the equine genome. Variants identified in MFM candidate genes, including two coding variants offered as commercial MFM equine genetic tests, did not associate with the WB MFM phenotype.Synthetic chalcogen-phosphorus chemistry permanently makes new challenges to computational NMR spectroscopy, which has proven to be a powerful tool of structural analysis of chalcogen-phosphorus compounds. This paper reports on the calculations of one-bond 31 P-77 Se and 31 P-125 Te NMR spin-spin coupling constants (SSCCs) in the series of phosphine selenides and tellurides. The applicability of the combined computational approach to the one-bond 31 P-77 Se and 31 P-125 Te SSCCs, incorporating the composite nonrelativistic scheme, built of high-accuracy correlated SOPPA (CC2) and CCSD methods and the DFT relativistic corrections (four-component level) was examined against the experiment and another scheme based on the four-component relativistic DFT method. A special J-oriented basis set (acv3z-J) for selenium and tellurium atoms, developed previously by the authors, was used throughout the NMR calculations in this work at the first time. The proposed computational methodologies (combined and "pure") provided a reaso

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