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https://www.selleckchem.com/products/pds-0330.html In this study, the secondary metabolites of a lichen-derived actinomycete strain Amycolatopsis sp. YIM 130687 were investigated intensively by using three different media (4#, 302#, and 312#) for fermentation. A total of 21 compounds were isolated from the fermented extraction of the strain. The structures of all compounds were identified by the examination of HRESIMS and NMR spectra. Compounds 1-3, 5, 6, 21 were only found in the cultivation on 302# medium, while compounds 4, 9-11 were only obtained when the strain was cultured on 312# medium. On the other hand, compounds 7, 8, and 20 were only isolated from the fermentation product on 4# medium. The antimicrobial activity test showed that compound 9 had significant inhibitory effects on bacterial pathogens of Staphylococcus aureus and MRSA with the MICs of 2 μg/ml and fungal pathogens of Botrytis cinerea and Fusarium graminearum with the MICs of 1 μg/ml.Plant growth promoting rhizobacteria (PGPR) are important for agriculture through their activity in stimulating and facilitating plant growth. The rhizobacteria were screened for molecular characterization and followed by their indole acetic acid (IAA) production, phosphate solubility, antibiosis activity. In this study, 162 soil samples were collected from the cocoa rhizosphere to isolate Bacillus subtilis strains using Mössel agar medium with an additional egg yolk and identified by sequencing the ytcP gene. The ability of each strain to form biofilms was obtained in a tube. Indole-3-acetic acid (IAA) production was estimated in Yeast Peptone Dextrose (YPB) broth. Phosphates were solubilized by each strain on Pikovskaya agar medium. The detection of lipopeptide genes using the molecular method has established the possession of isolates by antimicrobial genes. Fifty (50) B. subtilis strains were isolated and identified using the ytcP gene. Ninety percent (90%) of the strains were able to form a biofilm. All isolat

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