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https://www.selleckchem.com/products/fadraciclib.html further improvement.Besides serving in wastewater treatment and energy generation fields, electroactive biofilm (EAB) has been employed as a sensitive bio-elements in a biosensor to monitor water quality by delivering electrical signals without additional mediators. Increasing studies have applied EAB-based biosensor in specific pollutant detection, typically biochemical oxygen demand (BOD) detection, as well as in early-warning of composite pollutants. Based on a comprehensive review of literatures, this study reveals how EAB outputs electrical signal, how we can evaluate and improve this performance, and what information we can expect from EAB-based biosensor. Since BOD detection and early-warning are normally confusing, this study manages to differentiate these two applications through distinguished purposes and metrics. Based on the introductions of progresses and applications of EAB-based biosensors so far, several novel strategies toward the future development of EAB-based biosensors are proposed.Transcription factors (TFs) are the key proteins for the decision of cell fates, and they have been recognized as potent markers for diagnostic and treatment of diseases. Herein, we report on a highly sensitive biosensor for the detection of TFs based on the CRISPR/Cas12a system. This biosensor was accomplished based on the competitive binding of the Cas12a-crRNA and TFs towards a dsDNA referred to as activator. Without TFs, the activator can be recognized by Cas12a-crRNA and cause the activation of the DNase activity of Cas12a. When TFs were added, the TFs can bind with the activator because the activator was designed to contain the specific binding sites of target TFs. We find that this binding can inhibit the association between Cas12a-crRNA and the activator, which hinders the activation of Cas12a. As a proof-of-concept, the rapid detection of five kinds of TFs was presented, and the detection was extended to t

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