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https://www.selleckchem.com/products/ten-010.html Oligosaccharides analysis is crucial for brewing technology. Herein, we reported a rapid and highly reproducible method for profiling of oligosaccharides in beer using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) by employing a reasonably designed reactive-matrix, 2-phenyl-3-(p-aminophenyl) acrylonitrile (PAPAN). The PAPAN enhanced ionization efficiency of oligosaccharides and improved reproducibility comparing to the use of conventional matrix, 2,5-dihydroxybenzoic acid (DHB). After optimization of sample dilution factor and cationization agents, the distributions of maltooligosaccharides in different brands of beers were unambiguously identified. Since the PAPAN selectively reacts with the reducing end of oligosaccharides, the interferences from matrixes are effectively eliminated. Therefore, the method shows potentials for analysis of oligosaccharides in other foods. Covid19 has necessitated new methods of conducting outpatient consultations. At our institute, patient-facing paediatric ENT appointments were replaced with telemedical consultations. We performed a prospective analysis of the outcomes following telephone consultations with parents and describe our experience of remote consulting during a pandemic, and the possibilities for future applications of telemedicine in paediatric ENT. 215 patients were appropriately referred to the paediatric ENT clinic, 65% of these patients were deemed suitable for remote telephone consultation. Following a telephone call, 50% did not need further ENT clinic management, most commonly due to being listed for surgery (20%) or discharged (18%). The treating consultant assessed 81% of phone consultations as being effective. When given the choice, patients reported that 29% would choose a telephone consultation whilst 43% preferred a face to face consultation. Our experience has shown that there are areas of paediatric ENT that can

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