https://www.selleckchem.com/ Compared with methods that rely on optical three-dimensional interference microscope, the method proposed in this paper preserves the overall topography of leaf surface, and achieves superior cost performance at the same time. It is clear from experiments on standard gauge blocks that the RMSE of step was approximately 4.44 µm. Furthermore, according to the Friedman/Nemenyi test, the focus measure operator SML was expected to demonstrate the best performance. Compared with methods that rely on optical three-dimensional interference microscope, the method proposed in this paper preserves the overall topography of leaf surface, and achieves superior cost performance at the same time. It is clear from experiments on standard gauge blocks that the RMSE of step was approximately 4.44 µm. Furthermore, according to the Friedman/Nemenyi test, the focus measure operator SML was expected to demonstrate the best performance. We diagnosed a clinical case of pulmonary infection involving Mycobacteriumtuberculosis and Tropherymawhipplei in a patient with acute respiratory distress syndrome. The diagnosis was assisted by metagenomic next-generation sequencing of bronchoalveolar lavage fluid. A 44-year-old Han Chinese inmate was transferred to the emergency department because of dry cough, chest tightness, and shortness of breath. The patient's body temperature rose to 39.3°C following empirical cephalosporin treatment for 1week. The blood CD4+/CD8+ ratio was 0.7, suggesting immunodeficiency. Routine microbiological tests were performed, and tuberculosis interferon gamma release assays were positive. Mycobacteriumtuberculosis polymerase chain reaction was also positive. Chest computed tomography scan revealed miliary nodules and ground-glass opacifications, which were in accordance with tuberculosis. To fully examine the etiology, we performed routine laboratory tests and metagenomic sequencing, the results of which indicated the rone to infection by op