https://www.selleckchem.com/products/ots514.html Variation in these compounds was however not geographically specific, but most likely linked to the taste and aroma of the leaves. The relative abundance in glucosinolates and isothiocyanates in the leaves allowed the clustering of accessions into two main groups that could be used for further plant-herbivore interaction studies. This study revealed both the broad spectrum of phytochemicals present in Gynandropsis gynandra leaves and the substantial variation in metabolite profiles among accessions from different regions of the world. Our results provide a basis for the development of breeding programs aiming at improving the levels of specialised metabolites in this tropical leafy vegetable for increased resistance against pests and diseases and improved human health.Mucuna pruriens L., commonly known as velvetbean or cow-itch, is a self-pollinated tropical legume of the family Fabaceae, known for its medicinal properties. The active principle L-DOPA extracted from the plant is a potent drug used in the treatment of Parkinson's disease. Although, it is hypothesized that a single step reaction can produce L-DOPA, the presence of optional routes makes the pathway more intricate. For instance, the catecholamine biosynthetic pathway, which leads to L-DOPA production, could occur by hydroxylation of tyrosine to L-DOPA either by polyphenol oxidase (PPO) or tyrosine hydroxylase (TH). Furthermore, Cytochrome P450 (CYP) enzymes can also cause hydroxylation of tyrosine, resulting in L-DOPA synthesis. Therefore, the present investigation was focused on validating the step, which catalyzes the synthesis of L-DOPA, at the biochemical and molecular levels. Enzyme inhibitor studies showed significant inhibition of PPO enzyme with corresponding decrease in L-DOPA synthesis while TH and CYP inhibition had no effect on L-DOPA synthesis. Activity staining of non-denaturing PAGE gel for PPO and TH showed activity only to PPO enzyme. Fol