N2 is fixed as NH3 industrially by the Haber-Bosch process under harsh conditions, whereas biological nitrogen fixation is achieved under ambient conditions, which has prompted development of alternative methods to fix N2 catalyzed by transition metal molecular complexes. Since the early 21st century, catalytic conversion of N2 into NH3 under ambient conditions has been achieved by using molecular catalysts, and now H2O has been utilized as a proton source with turnover frequencies reaching the values found for biological nitrogen fixation. In this review, recent advances in the development of molecular catalysts for synthetic N2 fixation under ambient or mild conditions are summarized, and potential directions for future research are also discussed.Supported Ni catalysts are active in CO2 methanation. It is important to understand the reaction mechanism for the development of highly-active catalysts. In this study, we investigated the reaction pathways of CO2 methanation over Ni/Y2O3 and Ni/Al2O3 based on the adsorbates observed by diffuse reflectance infrared Fourier transform spectroscopy. For Ni/Al2O3, linear and bridged CO adsorbates were converted to nickel carbonyl hydride and/or formyl species, which would be further hydrogenated to methane. In contrast, the formation of formate adsorbates was specifically confirmed over Ni/Y2O3 under the CO2 methanation condition. The hydrogen molecule was activated by dissociatively-adsorbing on Ni particles. Then, the hydrogenation of formate adsorbates by the activated hydrogen species proceeded sequentially to form methane. The observed bridged CO species would not be a major intermediate for Ni/Y2O3.Breast cancer is the most common cancer in women and occurs mostly with poor outcomes. Our objective was to endow synthetic lethality to the phytoconstituent chikusetsusaponin IVa methyl ester (CSME, S), a special phytoconstituent from traditional Chinese medicine (TCM), Panax japonicus, with the photodynamic agent chlorin e6 (Ce6, C) and enhance the therapeutic efficacy against breast cancer using cell membrane-coated liposome nanoparticles (liposome, L). The delivery system based on liposomes was camouflaged by a hybrid cell membrane (RBC membrane and cancer cell membrane, M) and RGD (R) surface modifications to improve the solubility, targeting and treatment outcomes of CSME. Our results showed the successful development of nanocomplexes with extended half-life, increased immune evasion and targeted ability at the tumor site and good antitumor activity without side effects to normal tissue. The anti-tumor mechanism of nanocomplexes is related to cell proliferation regulation and apoptosis induction. Overall, this drug-delivery system provides a good alternative for breast cancer therapy using a natural active phytoconstituent.The osteonecrosis of femoral head (ONFH), a common refractory disease, is still not fully understood today. Hypoxia caused by ischemia is not only an important pathogenic factor but also a critical challenge for the survival of seed cells in the tissue engineering therapy of ONFH. To explore an efficient strategy to treat ONFH by targeting hypoxia, newly designed CaO2/gelatin microspheres were composited with 3D printed polycaprolactone/nano-hydroxyapatite (PCL/nHA) porous scaffold, sodium alginate/gelatin hydrogel, and bone marrow mesenchymal stem cells (BMSCs) to develop a novel tissue engineering scaffold and then transplanted into the core depression area of the ONFH rabbit model. The current data demonstrated that CaO2/gelatin microspheres can constantly release oxygen for 19 days. In vitro assays with BMSCs illustrated that scaffolds have high biocompatibility and are favorable for cell proliferation in extreme hypoxia (1% O2). The in vivo study demonstrated that the transplanted scaffold with oxygen-generating microspheres significantly enhanced the osteogenic and angiogenic effects compared to the scaffold without microspheres. Further assessments revealed that microspheres in the scaffold can reduce the local cell apoptosis and enhance the survival of grafted cells in the host. Collectively, the present study developed a novel oxygen slow-releasing composite scaffold, which can facilitate tissue engineering efficiency for treating the osteonecrosis of the femoral head by enhancing the angiogenesis and survival of grafted stem cells.
  To compare the load to failure values of different ceramic CAD/CAM implant crown materials with drilled screw access holes with and without cyclic loading applied.
 
  Forty zirconia abutments with a titanium base were pre-loaded onto implants to support maxillary right first premolar crowns that were milled from four different CAD/CAM ceramic materials (zirconia reinforced lithium silicate, hybrid ceramic, lithium disilicate, and zirconia; n = 10 each). After cementing the crowns, screw access channels were prepared by drilling through occlusal surfaces. Half of the specimens were subjected to cyclic loading for 5 million cycles at 2 Hz (n = 5/material).  https://www.selleckchem.com/products/ly-411575.html  After cyclic loading, vertical loads were applied to failure, and the load to failure values of all crowns were recorded and statistically analyzed. Two-way analysis of variance was used with restricted maximum likelihood estimation and Tukey-Kramer adjustments (α = .05).
 
  During cyclic loading, the zirconia abutment in one lithium disilicate specimen cracked at 2 million cycles, as well as a zirconia-reinforced lithium silicate crown. Results for the load to failure test series showed statistical differences between the materials. Zirconia resulted in significantly higher failure loads when compared to the other materials (P < .001). Cyclic loading did not significantly affect the load to failure values.
 
  Cyclic loading did not significantly influence the load to failure of any of the materials tested. Zirconia crowns with drilled screw access channels cemented on zirconia abutments with a titanium base had higher load to failure values compared to the other ceramic crown materials.
 Cyclic loading did not significantly influence the load to failure of any of the materials tested. Zirconia crowns with drilled screw access channels cemented on zirconia abutments with a titanium base had higher load to failure values compared to the other ceramic crown materials.