5 °C. A mild, eco-friendly chitin preparation method and an amino-monosaccharide content detection method of raw material before chitin preparation are described in this study, which can provide technical support for comprehensive utilization of Antarctic krill resources.Bacterial cellulose nanocrystals (BCNCs) were extracted from nata de coco waste and underwent sulphuric acid (H2SO4) hydrolysis for use as a reinforcement giving thermal and dimensional stability to polyether block amide (PEBAX) as a polymer matrix for the fabrication of BCNCs/PEBAX microporous membranes. The H2SO4-hydrolysis of BCNCs yielded rod-like/needle-like BCNCs and negatively charged surfaces, resulting from the generated surface sulfate groups on the bacterial cellulose (BC), which may be competent for numerous applications. The non-solvent induced phase separating (NIPS) and subsequent film casting methods were used to prepare the BCNCs/PEBAX microporous membranes. The obtained films were characterized with regards to their structure in terms of the content of crystalline phases, as well as their ionic transport and performance at elevated temperatures. The presence of the BCNCs fillers resulted in a good thermal and dimensional stability up to 150 °C and correlated with no membrane shrinkage. For NIPS membranes, the formation of a rigid cellulosic network within the matrix was emphasized and attributed to the thermal stabilization at temperatures above the melting temperature. In addition, the wettability, ionic conductivity, and thermal stability were investigated in BCNCs/PEBAX membranes filled with different amounts of BCNCs. Thus, the BCNCs/PEBAX membranes derived via NIPS had a remarkably good ionic conductivity, within the range of 10-2-10-3 S/cm, with up to 56.8% porosity. Such porous membranes are considered as an important and interesting candidate for the replacement of the commercial polyolefin-based microporous separator in lithium-ion batteries due to their superior electrochemical performances and the observed reinforcement effect.Chitosan is an abundant and renewable polysaccharide, and chemical modification can be used to enhance the biological properties of chitosan. In this study, monophenol and ortho-diphenol were introduced to chitosan through chemical modification to get chitosan derivatives owned high antioxidant activity. Their free radical-scavenging activity against three free radicals and reduction ability was tested. Based on the four antioxidant assay, the antioxidant ability of the synthesized chitosan derivatives exhibited a remarkable improvement over chitosan.  https://www.selleckchem.com/products/Sunitinib-Malate-(Sutent).html  The IC50 of inhibition of DPPH, hydroxyl (OH), and superoxide (O2-) radical-scavenging was 41-172, 10-89, and 14-38 μg/mL, respectively. Meanwhile, ortho-diphenol was more efficient group than monophenol on the improvement of antioxidant ability of chitosan derivatives. The possible free radical-scavenging mechanism, including the number of the hydroxyl groups and the presence ortho-dihydroxy substitution for the antioxidant ability were discussed. In vitro studies demonstrated that the constructed chitosan derivatives have good biocompatibility, and good antioxidant capacity to reduce oxidative stress. Functionalization of chitosan with phenolic group allows the possible applications on the therapy of oxidative-related diseases.The role exerted by the nucleus in the regulation of proteostasis in both health and disease is recognized of outmost importance, even though not fully understood. Many recent investigations are focused on its ability to modulate and coordinate protein quality control machineries in mammalian cells. Nucleophosmin 1 (NPM1) is one of the most abundant nucleolar proteins and its gene is mutated in ~30% of Acute Myeloid Leukemia (AML) patients. Mutations are localized in the C-terminal domain of the protein and cause cytoplasmatically delocalized and possibly aggregated forms of NPM1 (NPM1c+). Therapeutic interventions targeted on NPM1c+ are in demand and, to this end, deeper knowledge of NPM1c+ behavior in the blasts' cytosol is required. Here by means of complementary biophysical techniques we compared the conformational and aggregative behavior of the entire C-terminal domains of NPM1wt and type A NPM1c+ (bearing the most common mutation). Overall data show that only Cterm_mutA is able to form amyloid-like assemblies with fibrillar morphology and that the oligomers are toxic in human neuroblastoma SHSY cells. This study adds a novel piece of knowledge to the comprehension of the molecular roles exerted by cytoplasmatic NPM1c+ and suggests the exploitation of the amyloidogenic propensity of NPM1c+ as a new strategy for targeting AML with NPM1 mutations.Wound healing is a complex process involved in repairing tissue damage and preventing infection. However, there is a lack of appropriate treatment solutions that can simultaneously promote tissue repair and protect againstbacteria, especially antibiotic-resistant bacteria. In this study, we have developed an injectable hydrogel encapsulating acidic fibroblast growth factor (aFGF) and bacteriophage, termed as ABgel, for combating antibiotic-resistant bacteria and enhancing wound regeneration. ABgel is composed of oxidized sodium alginate (OSA), gelatin and hyaluronic acid (HA), and can rapidly form hydrogel with an elastic modulus of 13 kPa, which mimics the skin tissues. In addition, ABgel can effectively load and stabilize bacteriophage and aFGF, allowing for preventing bacterial infections and improving regeneration of damaged dermal tissues. In vitro studies demonstrate that ABgel exhibits enormous antibacterial activity against antibiotic-resistant E. coli and enhanced angiogenetic activity. Importantly, ABgel can promote skin regeneration and prevent bacterial infections in mice, thereby promoting wound healing process. Therefore, ABgel represents a decent bioactive engineered hydrogel dressing with a broad application potential.Due to the genotoxically challenging environments in which they live in, Mycobacteria have a complex DNA damage repair system that is governed by two major DNA damage responses, namely, the LexA/RecA-dependent response and the newly characterized PafBC-mediated response (Müller et al., 2018). The LexA/RecA-dependent response is a well-known bistable response found in different types of bacteria, and the Mycobacteria-specific PafBC-mediated response interacts with and modifies the LexA/RecA-dependent response (Müller et al., 2018). The interaction between the LexA/RecA-dependent response and the PafBC-mediated response has not been characterized mathematically. Our analysis shows that the addition of the PafBC-mediated response sensitizes the overall DNA damage response, effectively lowering the DNA damage rate threshold for activation.