https://www.selleckchem.com/products/ory-1001-rg-6016.html on behalf of the Botanical Society of America.Premise Pteroceltis tatarinowii (Ulmaceae), the only species of the genus Pteroceltis, is an endangered tree in China. Here, novel expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed to illuminate its genetic diversity for conservation and assisted breeding. Methods and Results Based on Illumina transcriptome data from P. tatarinowii, a total of 70 EST-SSR markers were initially designed and tested. Forty-eight of 70 loci (68.6%) were successfully amplified, of which 20 were polymorphic. The number of alleles per locus ranged from two to six, and the levels of observed and expected heterozygosity ranged from 0.018 to 0.781 and from 0.023 to 0.702, respectively. Additionally, cross-amplification was successful for 17 loci in two related species, Ulmus gaussenii and U. chenmoui. Conclusions These new EST-SSR markers are valuable transcriptomic resources for P. tatarinowii and will facilitate population genetics and molecular breeding of this species and its relatives in Ulmaceae. © 2020 Zhang et al. Applications in Plant Sciences is published by Wiley Periodicals, Inc. on behalf of the Botanical Society of America.Background Criterion-referenced cut-points for field-based aerobic fitness for children and adolescents are lacking. This study aimed to determine the associations between aerobic fitness and obesity to propose criterion-referenced cut-points for boys and girls (6-17 years). Methods A total of 61,465 children and adolescents aged 11.2 ± 2.0 years were recruited from 27 sites (all 26 states and Federal District) across Brazil. Aerobic fitness was assessed using 9-min walk/run test reported as distance attained during the test. Body mass index was calculated from measured height and weight and was used to identify obesity. Results The distance covered in the 9-min walk/run test (area under curve > 0.65) had satisfactory predictive