https://www.selleckchem.com/products/PD-0325901.html This study assessed animal welfare in ewes subjected to transcervical (TC) or laparotomy (LP) embryo collection, and the efficiency of these two techniques. Santa Inês ewes (n = 57) received a protocol for estrus synchronization and superovulation. Cervical dilation protocol was initiated 12 h before embryo collection in all ewes. Depending on the success of cervical passage, the embryos were collected from ewes by either TC or LP. Records were made of physiological (rectal temperature (RT) and heart rate (HR)), endocrine (cortisol concentration), biochemical (glycaemia, total proteins, globulin and albumin concentrations), and behavioral variables. Data were recorded before fasting (BF) and sedation (BS), during (DC) and immediately after embryo collection (IAC), and 1 h (1hAC), 3 h (3hAC), 6 h (6hAC), 12 h (12hAC), 24 h (24hAC), and 48 h (48hAC) after embryo collection. The LP and TC procedures were applied to 22 and 35 ewes (with 100.0% and 94.3% of procedures being successful, respectively). The use of LPhe TC procedure is probably less stressor than the LP.The environmental pollutants with hormonal activities may influence steroid-mediated processes in neonatal ovaries and increase the incidence of reproductive disorders. The aim of the current study was to examine effects of 4-tert-octylphenol (OP), a non-ionic surfactant widely used in a variety of industrial applications which has been reported to mimic the 17β-estradiol activity, on the expression of protein-coding (mRNAs) and long non-coding (lncRNAs) transcripts in neonatal ovaries of the pig. By employing RNA-Seq we aimed to gain insights into regulatory networks underlying the OP effects on the follicular development in pigs. Piglets were injected (sc) daily with OP (100 mg/kg bw) or corn oil (controls) between postnatal Days 1 and 10 (n = 3/group). Ovaries were excised from the 11-day-old piglets and total cellular RNA was isolated and sequenced. Two