Baculovirus-infected larvae release progeny viral occlusion bodies (OBs) to enable cyclical virus transmission to new hosts. The alphabaculovirus chitinase and cathepsin enzymes cause terminal liquefaction of host insect cadavers, aiding OB dispersal. The mechanism of cell lysis required to release the OBs is unclear but here we show Autographa californica multiple nucleopolyhedrovirus cathepsin protease activity is required for efficient release of the host tissue-degrading chitinase and cathepsin enzymes and critical for release of progeny OBs from virus-infected cells. Comparisons between viruses containing or lacking cathepsin indicate that cathepsin was necessary for OB release into cultured cell media or hemolymph of insects. In addition, pharmacological inhibition of cysteine protease activity in cells during infection blocked maturation of active cathepsin and OB release from infected cells. Together, these results suggest an important link between baculovirus-induced cell lysis, the concomitant maturation of cathepsin, and cellular release of chitinase, cathepsin and progeny OBs from cells.The transformation that pharmaceuticals can undergo during the water reclamation cycle, or by biotic/abiotic reactions when reclaimed water (RW) is used for irrigation, can lead to the presence of transformation products (TPs) in agricultural environments. However, data on TPs in real crops are scarce. Herein, a suspect screening approach was applied for the comprehensive investigation of 262 potential TPs, associated with 20 prioritised pharmaceuticals found in real tomato crops exposed to long-term RW irrigation. The occurrence and fate of the TPs was evaluated by the retrospective analysis of RW, soil, leave and tomato samples from 4 intensive production greenhouses. Sample analysis was accomplished by liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (LC-QTOF-MS). Up to 18 TPs were tentatively identified, of which 2 were not previously reported. 7 TPs were finally confirmed with analytical standards. 5 TPs were determined in RW, 15 TPs in soil and 2 TPs in leaves. Remarkably, the investigated TPs were not found in tomato fruits. These results shed light on the variety of TPs that can be found in the water reuse cycle and contribute to the assessment of the global risks of wastewater reuse and the safety of the vegetable and fruit production system.A variety of metal elements have exhibited strong reductive and dehalogenative capabilities for the removal of persistent organic pollutants, owing to electron transfer or electron-hole activation through various methods. Herein, a bimetallic CNi-Al2O3 structure (AlCNi) was successfully synthesized to simultaneously function as sorbent and catalyst in the reduction of perfluoroalkyl carboxylic acids (PFOA) polluted wastewater. Using a reaction period of 3 h, 98% of PFOA was removed by AlCNi through a mechanochemical stirring method and 70.43% of fluorine ions was released from PFOA anchored onto the surface of AlCNi. Both thermocatalysis and photocatalysis technologies were incorporated and compared when utilized in tandem with AlCNi to mitigate the PFOA. In addition, peroxymonosulfate (PMS) and sodium sulfite (Na2SO3) were also integrated into experiments, separately, as a strong oxidant and reductant to improve the degradation effect of PFOA. However, the degradation efficiency of both were lower than that of AlCNi, even when assisted by elevated temperatures and ultraviolet irradiation. The feasibility of employing AlCNi for PFOA degradation was further investigated at various temperature and pH conditions. The data obtained from HPLC-MS/MS, TOC, and IC with multiple characterizations of AlCNi/PFOA, proposed the predominant degradation pathways comprising adsorption, defluorination-hydroxylation, and decarboxylation. This study provides a valuable remediation method without utilizing chemical agents and special activation for PFOA by AlCNi, which can be suitable for large-scale sewage treatment applications.Novel waste-derived sorbents synthesized through one-step co-pyrolysis of wood and PVC (or brominated flame retarded plastic) were demonstrated as cost-effective sorbents for mercury (Hg) removal in our previous studies.  https://www.selleckchem.com/products/Sunitinib-Malate-(Sutent).html  To introduce magnetism and improve porosity, Fe species were further doped into such waste-derived sorbents. The ultimate fate of Hg-laden sorbents after their service is mainly disposed in landfill. Therefore, the stability of Hg/halogens on the spent sorbents is an important topic. In this work, the leachability of Hg/Cl/Br from four waste-derived sorbents was evaluated using toxicity characteristic leaching procedure (TCLP). Three traditional sorbents (Cl-impregnated activated carbon, Br-impregnated activated carbon and commercial activated carbon) were also tested for comparison. Experimental results suggested that the stability of Hg/Cl/Br on four waste-derived sorbents was far higher than that prepared by chemical impregnation. For four waste-derived sorbents, little Hg was leached out whereas certain amounts of Cl/Br escaped into the leachate. Interestingly, Fe-doping effectively improved the stability of Hg/Cl/Br on the waste-derived sorbents. Kinetic analysis revealed that diffusion process and surface chemical reaction were respectively the rate-limiting step for waste-derived sorbents before and after Fe-doping. Water-washing pretreatment could remove loosely-bonded Cl/Br from the waste-derived sorbents, while the Cl/Br essential for Hg removal was retained.Bioaugmentation methods are frequently employed for pesticide pollution remediation; however, it is not clear whether the introduced bacteria affect the pesticide bound residue (BRs) composition and whether the BRs can be catabolized by the introduced strains. This study aimed at answering these questions by using 14C-chlorpyrifos (14C-CPF) and two CPF-degrading strains (Pseudomonas sp. DSP-1 and Cupriavidus sp. P2). The results showed that the BRs can be up to 83.0%, and that the CPF-BRs formed can be further transformed into 14CO2 by the strains. Indeed, the microbial inoculation can increase the CPF mineralization by 1.0-22.1 times and can decrease the BRs by up to ~50% of the control (on day 20). Compared with the control without bioaugmentation, microbial inoculation enhanced the release of BRs by 2.2-18.0 times. Adding biochar to the soil can greatly inhibit CPF mineralization and maintain the BR content at a relatively stable level. The CPF residue can affect the composition of the indigenous soil microbial community, but the introduction of bacteria for remediation did not have a significant effect.