β-carotene is an efficient antioxidant and its accumulation is an oxidative response to stressors. Dunaliella salina strain GY-H13 is rich in β-carotene under environmental stresses, which was selected as material to understand the molecular mechanism underlying β-carotene biosynthesis. Seven full length cDNA sequences in β-carotene biosynthesis pathway were cloned, including geranylgeranyl pyrophosphate synthase (GGPS), phytoene synthase (PSY), phytoene desaturase (PDS), 15-cis-zeta-carotene isomerase (ZISO), zeta-carotene desaturase (ZDS), prolycopene isomerase (CRTISO), lycopene beta-cyclase (LCYb). The seven protein sequences from the strain GY-H13 showed the highest similarity with other D. salina strains. Especially, PSY, PDS and LCYb protein sequences shared 100 % identity. Phylogenetic analysis indicated all proteins from GY-H13 firstly clustered with those from other D. salina strains with a bootstrap of 100 %. Multiple alignment indicated several distinct conserved motifs such as aspartate-rich domanant of the increased accumulation of β-carotene in microalgae, which help their survive under harsh environments. The newly isolated D. salina strain GY-H13 would be a promising microalgae model for investigating the molecular mechanism of stress-induced β-carotene biosynthesis. Due to some special characteristics like the effective thermal conductivities, appropriate mechanical features, and superior electrical properties, carbon nanostructures have been known as the proper materials to reach the desired characteristics of fluids. In the recent past fluid flows through peristaltic mechanism subject to carbon nanotubes are utilized to handle the overcome of industrial and physiological materials thermal properties. Due to rich thermal characteristics nanotubes are used into basic industrial materials to improve the required ability of thermal properties of these industrial materials. Thus various kinds of nanoparticles e.g.  https://www.selleckchem.com/products/Azacitidine(Vidaza).html  aluminum, copper, zinc oxides and carbon nanotubes are significantly utilized to increase the thermal abilities of base liquids. Because of the several significant special qualities such as improved thermal conductivities, applicable mechanical structures, and rich electrical properties, CNTs have been acknowledged as the accurate tools to reach the wanted featureorption case and enhances for heat generation case. Impact of Prandtl number on entropy indicates that entropy is minimum due to less fluid friction (i.e. Prandtl number less than 1). OBJECTIVE Herein, a neural network-based liver segmentation algorithm is proposed, and its performance was evaluated using abdominal computed tomography (CT) images. METHODS A fully convolutional network was developed to overcome the volumetric image segmentation problem. To guide a neural network to accurately delineate a target liver object, the network was deeply supervised by applying the adaptive self-supervision scheme to derive the essential contour, which acted as a complement with the global shape. The discriminative contour, shape, and deep features were internally merged for the segmentation results. RESULTS AND CONCLUSION 160 abdominal CT images were used for training and validation. The quantitative evaluation of the proposed network was performed through an eight-fold cross-validation. The result showed that the method, which uses the contour feature, segmented the liver more accurately than the state-of-the-art with a 2.13% improvement in the dice score. SIGNIFICANCE In this study, a new framework was introduced to guide a neural network and learn complementary contour features. The proposed neural network demonstrates that the guided contour features can significantly improve the performance of the segmentation task. Methamphetamine (METH) has been reported to induce endoplasmic reticulum (ER) stress and neuronal apoptosis in the central nervous system (CNS) during the development of addiction. Thioredoxin-1 (Trx-1) is a redox regulating protein and plays an important role in inhibiting apoptosis and protects neurons from cytotoxicity through ER and mitochondria-mediated pathways. Our previous study has been reported that Trx-1 protects mice from METH-induced rewarding effect. However, whether Trx-1 plays the role in resisting METH injury is still unclear. Here, we aim to investigate whether Trx-1 participates in the regulation of METH-induced CNS injury via ER stress and mitochondria-mediated pathways. Our study first repeated the contioned place preference expression induced by METH. Then we detected and found that METH increased the expression of N-methyl-d-asparate (NMDA) receptor subunit 2B (NR2B) and the level of glutamate (Glu) in the ventral tegmental area (VTA) and nucleus accumbens (NAc), while Trx-1 overexpression suppressed the increases. We further examined ER stress-related proteins and mitochondrial apoptosis pathway in the VTA and NAc, and found that METH increased the expressions of glucose regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), and Bax, as same time decreased the expressions of procaspase12, Bcl-2, and procaspase3, while Trx-1 overexpression blocked these changes. These results indicate that Trx-1 blocks METH-induced injury by suppressing ER stress and mitochondria-mediated apoptosis in the VTA and NAc via targeting glutamatergic system. The discovery of the TLRs family and more precisely its functions opened a variety of gates to modulate immunological host responses. TLRs 7/8 are located in the endosomal compartment and activate a specific signaling pathway in a MyD88-dependant manner. According to their involvement into various autoimmune, inflammatory and malignant diseases, researchers have designed diverse TLRs 7/8 ligands able to boost or block the inherent signal transduction. These modulators are often small synthetic compounds and most act as agonists and to a much lesser extent as antagonists. Some of them have reached preclinical and clinical trials, and only one has been approved by the FDA and EMA, imiquimod. The key to the success of these modulators probably lies in their combination with other therapies as recently demonstrated. We gather in this review more than 360 scientific publications, reviews and patents, relating the extensive work carried out by researchers on the design of TLRs 7/8 modulators, which are classified firstly by their biological activities (agonist or antagonist) and then by their chemical structures, which total syntheses are not discussed here.