s.Hg accumulation in marine organisms depends strongly on in situ water or sediment biogeochemistry and levels of Hg pollution. To predict the rates of Hg exposure in human communities, it is important to understand Hg assimilation and processing within commercially harvested marine fish, like the European seabass Dicentrarchus labrax. Previously, values of Δ199Hg and δ202Hg in muscle tissue successfully discriminated between seven populations of European seabass. In the present study, a multi-tissue approach was developed to assess the underlying processes behind such discrimination. We determined total Hg content (THg), the proportion of monomethyl-Hg (%MeHg), and Hg isotopic composition (e.g. Δ199Hg and δ202Hg) in seabass liver. We compared this to the previously published data on muscle tissue and local anthropogenic Hg inputs. The first important finding of this study showed an increase of both %MeHg and δ202Hg values in muscle compared to liver in all populations, suggesting the occurrence of internal MeHg demethylation in seabass. This is the first evidence of such a process occurring in this species. Values for mass-dependent (MDF, δ202Hg) and mass-independent (MIF, Δ199Hg) isotopic fractionation in liver and muscle accorded with data observed in estuarine fish (MDF, 0-1‰ and MIF, 0-0.7‰). Black Sea seabass stood out from other regions, presenting higher MIF values (≈1.5‰) in muscle and very low MDF (≈-1‰) in liver. This second finding suggests that under low Hg bioaccumulation, Hg isotopic composition may allow the detection of a shift in the habitat use of juvenile fish, such as for first-year Black Sea seabass. Our study supports the multi-tissue approach as a valid tool for refining the analysis of Hg sourcing and metabolism in a marine fish. The study's major outcome indicates that Hg levels of pollution and fish foraging location are the main factors influencing Hg species accumulation and isotopic fractionation in the organisms.Air pollution caused by PM2.5 particles is a critical issue for public health that adversely affects people living in urban cities. Short-term Mega-events such as international meetings, sports tournaments, and traditional festivals can profoundly influence the local air quality. However, the extent of these influences and their role in improving or deteriorating the local air quality is still unclear. By collecting relative research from 75 publications based on more than 37 cities worldwide, we conducted a systematic review and meta-analysis. We calculated the log response ratio (RR) of the treatment (during) and control periods (before and after) of the Mega-events. The short-term policy control measures enacted during the Mega-Events consisting of meetings caused a significant decline (by -44.06%) in the ambient PM2.5 concentration. The mean daily PM2.5 concentration reduced from more than 100.00 μg/m3 before the events to 60.39 μg/m3, which is below the WHO (World Health Organization) interim target - 1 (75 μg/m3). On the contrary, setting off fireworks during the festival increased the ambient PM2.5 concentrations by 89.57% on average, with a mean daily value of 254.22 μg/m3. The variations in the effects of all event types on the air quality were primarily influenced by the background PM2.5 concentrations, with a negative correlation throughout. Moreover, the impact of events with policy control measures was also influenced by the year of the event, level of control, and location (suburban/urban) of the monitoring sites. Our findings provide evidence of the potential of human intervention on PM2.5 pollution reduction. We further highlight the crucial role of background pollution level in implementing policies during the Mega-events, which can benefit the environmental governance of developing countries.Although the occurrence and distribution of various chemicals on microplastics (MPs) has been widely studied, little was known about the concentrations of poly- and perfluoroalkyl substances (PFASs) on MPs.  https://www.selleckchem.com/products/larotrectinib.html  In this study, MPs from eight rivers draining into Pearl River Estuary (PRE) region were collected and analyzed. Higher concentrations of PFASs on MPs (105-9.07 × 103 ng g-1) were found in the drain outlets receiving wastewater from most urbanized cities with large population densities. On the other hand, lower concentrations of PFASs on MPs (10.3-227.8 ng g-1) were found in the drain outlets receiving wastewater mostly from agricultural and forested areas. Specially, 82 disubstituted polyfluoroalkyl phosphates (82 diPAP) was detected with the highest frequency, in 92.5% of the samples. Furthermore, a positive Spearman correlation was found between 62 disubstituted polyfluoroalkyl phosphates (62 diPAP) and perfluorotetradecanoic acid (PFTeDA) (rs = 0.621, p = 0.012), indicating they might share similar sources. PFASs on MPs were found to vary significantly with different seasons. Higher concentrations of PFASs on MPs were found in dry seasons, while lower concentrations were observed in wet seasons. The results of this study should be important for the understanding of PFAS occurrence and distribution on MPs and the partitioning mechanism of PFASs on MPs in estuary systems.Artificial light at night (ALAN) is a major driver of firefly population declines, but its physiological effects are not well understood. To investigate the impact of ALAN on firefly development, we exposed larval Aquatica ficta fireflies to ALAN for two weeks. High larval mortality was observed in the periods of 1-68 days and 106-134 days post-treatment, which may represent the short- and long-term impacts of ALAN. We then profiled the transcriptome of larval Aquatica ficta fireflies following two weeks of ALAN exposure. A total of 1262 (1.67% out of 75777 unigenes) were differentially expressed in the treatment group 1157 were down-regulated, and 105 were up-regulated. Up-regulated unigenes were related to regulation of hormone levels, ecdysteroid metabolic process, and response to stimulus; down-regulated unigenes were related to negative regulation of insulin receptor signaling, germ cell development, oogenesis, spermatid development, and regulation of neuron differentiation. Transcriptome results suggest that the endocrine, reproductive, and neural development of firefly larvae could be impaired by even relatively brief period of ALAN exposure.