91 and 3.06 µg/mL for TRG, 0.99, and 3.30 µg/mL for DI and 0.33 and 1.10 µg/mL for NA. The validated method was applied for determination of the analytes in the tablet, capsule and thin film dosage forms prepared from the fenugreek seed extract. The mean recovery percentages of the analytes were in the range of 90.0-97.4%, 85.6-105.4%, and 90.0-99.0% for tablet, capsule, and film dosage forms, respectively. Generally, the validated method could be a good candidate for routine spectrophotometric determination of the analytes without any necessity for pre-analysis extraction.Prognosis of metastatic breast cancer is very poor which urges the necessity to develop novel potential drug candidates. We assessed two compounds with tri-aryl structures (A and B) for their potency to reduce primary breast tumor growth and lung metastasis in 4T1 mice model. MTT assay, 4T1 mammary mouse model, and immunohistochemistry experiments were used in this study. In-vitro results exhibited an anti-proliferative effect for compounds A and B towards MDA-MB-231 cancer cells. Our in-vivo results displayed that administered compounds A and B could suppress the size of the primary tumor and the number of lung metastatic foci in 4T1 BALB/c mice model. Histopathological analysis revealed that treatment of both compounds resulted in necrosis. Our findings provide new evidence that compound B may be promising for slowing the growth of tumor along with metastatic foci via COX-2 independent pathway.This study aimed to improve delivery of lomustine as a chemotherapeutic agent and to increase its uptake by U87-MG cancer cells via synthesizes LN-FA-PG-SPIONs (lomustine loaded polyglycerol coated superparamagnetic iron oxide nanoparticles conjugated with folic acid). Nanoparticles were synthesized by thermal decomposition method and characterized using TEM (transmission microscope), FTIR (Fourier transform infrared spectroscopy), and VSM (vibrating sample magnetometer). Lomustine release from nanoparticles was determined by dialysis-bag diffusion technique. Nanoparticles cytotoxicity was evaluated by MTT assay. Mean size of SPIONs and FA-PG-SPIONs (PG-SPIONs conjugated with folic acid) were 7.1 ± 1.13 nm and 25.1 ± 3.94 nm, respectively. Based on FTIR spectra SPIONs were successfully coated by polyglycerol and conjugated with folic acid. Lomustine encapsulation efficiency was 46 ± 6.8 %. SPIONs were cytotoxic on U87-MG cells at concentration above 100 ug/ml (p 0.05). Conjugation of folic acid with PG-SPIONs increased nanoparticles uptake by U87-MG cells (p less then 0.05). We concluded that however FA-PG-SPIONs are proposed as a useful tracer for diagnostic and treatment of GBM but their drug delivery properties for lomustine is not satisfactory and more researches are necessary with this regard.Transdermal patches loaded with pravastatin was previously characterized in another published study by Serrano-Castañeda et al; 2015. These transdermal patches (TP) were generated by the plate casting technique, the in-vitro percutaneous absorption studies of TP were evaluated for three different formulations with different quantities of Pluronic F-127 (PF-127) i) without PF-127 (TP W), ii) 1% of PF-127 (TP 1%), and iii) 3% of PF-127 (TP 3%) using solid microneedles as a penetration enhancer with two different lengths i) 0.25 mm and ii) 2.25 mm and iii) in-vitro permeation studies by passive diffusion. The fluxes (F), time lag (tLag) and permeability constants (Kp) for each formulation were TP W (F38.5µg/cm2*h, tLag18.97h and Kp5.9x10-3 cm/h), TP W with microneedles of 0.25 mm (F103.3 µg/cm2*h, tLag 20.76 h and Kp 0.0158 cm/h), TP W and microneedles of 2.25 mm (F105.2µg/cm2*h, tLag 21.16 h and Kp 0.0159cm/h), TP 1% (F90 µg/cm2*h, tLag 19.48 h and Kp 0.0137 cm/h), TP 1% with microneedles of 0.25 mm (F111.4µg/cm2*h, tLag19.11h and Kp0.017cm/h), and TP 1% with microneedles of 2.25 mm (F115.2µg/cm2*h, tLag16.73h and Kp0.017cm/h), TP 3% (F40.9µg/cm2*h, tLag20.45h and Kp0.0062 cm/h), TP 3% with microneedles of 0.25 mm (F67.1 µg/cm2*h, tLag 21.79h and Kp0.0102cm/h) and TP 3% with microneedles of 2.25 (F70.5 µg/cm2*h, tLag20.44h and Kp0.0107cm/h). Results show that the formulation of TP affects the pravastatina flux and Kp parameters, however the length of microneedles only has important effect on tLag.Biosurfactants, the microbial originated surface active agents, can modify the physicochemical properties of surfaces and reduce the bacterial adhesion via changing bacterial adhesion interactions on surfaces. They were also able to block oxidative chain reactions and might show antioxidant properties. The goal of this study was to evaluate the antioxidant and antibiofilm activities of biosurfactants which were derived from two autochthonous biosurfactant-producing strains, Bacillus amyloliquefaciens NS6 (surfactin), and Pseudomonas aeruginosa MN1 (rhamnolipids). Their antioxidant activities were determined by ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) methods. Ferric thiocyanate (FTC) assay was used for determination of their lipid peroxidation inhibition capacity. Their effect to reduce the adhesion of Streptococcus mutans on polystyrene surfaces and disruption of its pre-formed biofilms were also investigated. Our results indicated that surfactin showed higher antioxidant activity than rhamnolipids and showed relatively similar efficiency to BHA that suggests it as a good alternative for synthetic antioxidants. In other hand, rhamnolipid conditioned surfaces showed higher antiadhesive and antibiofilm activity in comparison with surfactin treated surfaces.One of the newest methods to reduce cerebral ischemia damages is cell therapy. The aim of this study is to evaluate the effect of Sertoli cell transplantation on ischemia-induced injuries in animal models of stroke. Rats were divided into four groups transplant+ischemia, ischemia, sham, and control. Sertoli cells were separated from the other testis of rats and cultured.  https://www.selleckchem.com/products/VX-770.html  Unilateral Sertoli cell transplantation was performed in the right striatum by using stereotaxic surgery. For induction of brain ischemia, middle cerebral artery occlusion surgery was used 14 days after transplantation. By using western blotting method, expression of nuclear factor kappa (NF-кB) and Bax were evaluated. In this study, a remarkable decrease in neurological deficits, infection, blood-brain barrier permeability, and brain edema was observed in the cell transplant recipient group in comparison with the ischemia group. Probably, a reduction in inflammation (NF-кB factor) and apoptosis (Bax) following injection of Sertoli cells result in amelioration of ischemic damages induced by MCAO surgery.