melanogaster populations. The crayfish ingestion in some population of T. melanogaster can be explained by environmental differences between populations, or by recent origin of crayfish ingestion in T. melanogaster. ©2020 Manjarrez et al.Background This study was performed to determine the characteristics of community cats that were admitted to trap-neuter-return(TNR) programs and the feasibility of the high-quality, high-volume spay/neuter (HQHVSN) model in Seoul, Korea. Methods TNR programs were performed eight times from 2017 to 2018, and a total of 375 community cats from the pilot areas were admitted. The pilot areas were selected regions wherein caregivers voluntarily participated in our TNR program. Each cat was anesthetized, assessed for health status, sterilized, vaccinated against feline viral rhinotracheitis, calicivirus and panleukopenia (FVR-CP), and rabies, and treated with insecticide after surgery. The time from anesthesia to recovery was evaluated to verify the efficiency of surgical time of the program. The TNR program at a local animal hospital and the program in this study were compared to assess the veterinary treatment administered and the cost for each cat. Results A total of 375 cats were underwent TNR in this study, i effectively apply HQHVSN clinics in Korea. Future studies that perform intensive sterilization in targeted areas are necessary to confirm the efficacy of the TNR strategy. ©2020 Cho et al.Background The invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) is a polyphagous pest that causes widespread damage particularly to maize and sorghum in Africa. The microbiome associated with S. frugiperda could play a role in the insects' success and adaptability. However, bacterial communities in S. frugiperda remain poorly studied. Methods We investigated the composition, abundance and diversity of microbiomes associated with larval and adult specimens of S. frugiperda collected from four maize growing regions in Kenya through high throughput sequencing of the bacterial 16S rRNA gene.  https://www.selleckchem.com/products/acetylcysteine.html  The population structure of S. frugiperda in Kenya was assessed through amplification of the mitochondrial cytochrome oxidase subunit I gene. Results We identified Proteobacteria and Firmicutes as the most dominant bacterial phyla and lesser proportions of Bacteroidetes and Actinobacteria. We also observed differences in bacterial microbiome diversity between larvae and adults that are a likely indication that some prominent larval bacterial groups are lost during metamorphosis. However, several bacterial groups were found in both adults and larvae suggesting that they are transmitted across developmental stages. Reads corresponding to several known entomopathogenic bacterial clades as well as the fungal entomopathogen, Metarhizium rileyi, were observed. Mitochondrial DNA haplotyping of the S. frugiperda population in Kenya indicated the presence of both "Rice" and "Corn" strains, with a higher prevalence of the "Rice" strain. © 2020 Gichuhi et al.The aim of this study was to compare the effect of self-selected repetition duration (SELF), with and without volume load (VL) equalized with controlled repetition duration (CON) on muscle strength and hypertrophy in untrained males. We used a within-subjects design in which 20 volunteers (age 24.7 ± 2.9 years) had one leg randomly assigned to CON (i.e., 2 s concentric, 2 s eccentric) and the other to SELF or to self-selected repetition duration with equalized volume load (SELF-EV). One repetition maximum (1-RM) and muscle cross-sectional area (CSA) were measured at baseline (Pre) and after (Post) resistance training (RT; 2×/wk for 8 weeks). For the main study variables (1-RM and muscle CSA), a mixed-model analysis was performed, assuming repetition duration (SELF, SELF-EV and CON), and time (Pre and Post) as fixed factors and the subjects as random factor for each dependent variable (1-RM and CSA). All RT protocols showed significant increases in values of 1-RM from Pre (CON 73.7 ± 17.6 kg; SELF 75.9 ± 17.7 kg; and SELF-EV 72.6 ± 16.9 kg) to Post (CON 83.4 ± 19.9 kg, effect size (ES) 0.47; SELF 84 ± 19.1 kg, ES 0.43; and SELF-EV 83.2 ± 19.9 kg, ES 0.57, P 0.05). Performing RT with SELF, regardless of VL, was equally effective in inducing increases in muscle strength and hypertrophy compared to CON in untrained men. © 2020 Chaves et al.The Flos Lonicerae Japonicae (FLJ), Lonicera japonica Thunb, belonging to the Caprifoliaceae family, is an economically important plant that is highly utilized in traditional Chinese medicine as well as in Japanese medicine. The flowers of these plants are rich in chlorogenic acid (CGA) and luteoloside. Our previous study revealed that tetraploid L. japonica has higher fresh/dry weight, phenolic acids and flavonoids contents than those of diploid plants. However, why tetraploid L. japonica can yield higher CGA and luteolosides than that in diploid and what is the difference in the molecular regulatory mechanism of these pathways between diploid and tetraploids remained unclear. Therefore, in the present study, we performed comprehensive transcriptome analyses of different flowering stages of diploid and tetraploid L. japonica. The CGA content of tetraploid was found higher than that of diploid at all the growth stages. While the luteoloside content of diploid was higher than that of tetraploid at S4 and S6 growth stages. We obtained a high-quality transcriptome assembly (N50 = 2,055 bp; Average length = 1,331 bp) compared to earlier studies. Differential expression analysis revealed that several important genes involving in plant hormone signal transduction, carbon metabolism, starch/sucrose metabolism and plant-pathogen interaction were upregulated in tetraploid compared with the diploid L. japonica, reflecting the higher adaptability and resistance of tetraploid species. Furthermore, by associating the phenotypic data and gene expression profiles, we were able to characterize the potential molecular regulatory mechanism of important biosynthetic pathways at different flowering stages. Overall, our work provides a foundation for further research on these important secondary metabolite pathways and their implications in traditional Chinese/Japanese medicine. © 2020 Wang et al.