Low polar 12-(His)4-12 aggregates into long fibers, which are very difficult to dissociate and they mainly electrostatically bind on the negative microbial surface, resulting in its weakest antimicrobial and antibiofilm activity. This study reveals the effect of the antimicrobial peptide structure and aggregation on the antimicrobial activities and would be helpful for developing high-efficient antimicrobial peptides with antibiofilm activity.Metal-organic frameworks (MOFs) are a class of porous materials with versatile properties. In this study, ZIF-8 was employed to establish a two-enzyme system by encapsulating permeabilized Bacillus subtilis cells coated with glucose isomerase. B. subtilis was constructed by introducing the shuttle plasmid PMA5 associated with the overexpression of trehalose synthase. Using this two-enzyme system, trehalose was produced by trehalose synthase and the byproduct glucose was converted to fructose with the help of glucose isomerase. The decrease in glucose production not only relieved the inhibition of the entire reaction chain but also increased the final yield of trehalose. The highest trehalose production rate reached 67.7% and remained above 50% after 20 batches. In addition, the toxicity of the ZIF-8 coating for B. subtilis was investigated by fluorescence microscopy and was found to be negligible. By simulating an extreme environment, the ZIF-8 coating was demonstrated to have a protective effect on the cells and enzymes. This study provides a theoretical basis for the application of MOFs in the immobilization of microorganisms and enzymes.Fourteen new compounds, oudemansins 1-4, oudemansinols 5-7, favolasins 8-10, favolasinin (12), polyketides 13-15, and (R,E)-2,4-dimethyl-5-phenyl-4-pentene-2,3-diol (16), together with nine known compounds were isolated from the basidiomycete fungus Favolaschia sp. BCC 18686. Two new compounds, favolasin E (11) and 9-oxostrobilurin E (17), were isolated from the closely related organism Favolaschia calocera BCC 36684 along with nine β-methoxyacrylate-type derivatives. Compounds in the class of oudemansins and strobilurins exhibited moderate to strong antimalarial activity with relatively low cytotoxicity against Vero cells (African green monkey kidney fibroblasts). Potent antimalarial activity was demonstrated for 9-methoxystrobilurins G, K, and E (IC50 values 0.061, 0.089, and 0.14 μM, respectively). The structure-activity relationships (SAR) for antimalarial activity is proposed on the basis of the activity of the new and several known β-methoxyacrylate derivatives in combination with the data from previously isolated compounds. Furthermore, several compounds showed specific cytotoxicity against NCI-187 cells (human small-cell lung cancer), although the SAR was different from that for antimalarial activity.As atmospheric levels of carbon dioxide (CO2) continue to increase, there is an immediate need to balance the carbon cycle. Current approaches require multiple processes to fix CO2 from the atmosphere or flue gas and then reduce it to value-added products. The zinc(II) catalyst Zn(DMTH) (DMTH = diacetyl-2-(4-methyl-3-thiosemicarbazonate)-3-(2-pyridinehydrazonato)) reduces CO2 from air to formate with a faradaic efficiency of 15.1% based on total charge. The catalyst utilizes metal-ligand cooperativity and redox-active ligands to fix, activate, and reduce CO2. This approach provides a new strategy that incorporates sustainable earth-abundant metals that are oxygen and water tolerant.White organic light-emitting diodes (WOLEDs) using thermally activated delayed fluorescence (TADF)-based single emissive layer (SEL) have attracted enormous attention because of their simple device structure and full exciton utilization potential for high efficiency. However, WOLEDs made of an all-TADF SEL usually exhibit serious efficiency roll-off and poor color stability due to serious exciton-annihilation and unbalanced radiative decays of different TADF emitters. Herein, a new strategy is proposed to manipulate the TADF-sensitized fluorescence process by combining dual-host systems of high triplet energy with a conventional fluorescent emitter of complementary color. The multiple energy-funneling paths are modulated and short-range Dexter energy transfer is largely suppressed due to the steric effect of peripheral tert-butyl group in the blue TADF sensitizer. The resulting all-fluorescent WOLEDs achieve an unprecedentedly high external quantum efficiency of 21.8% with balanced white emission of Commission Internationale de l'Eclairage coordinate of (0.292, 0.343), accompanied with good color stability, reduced efficiency roll-off, and prolonged operational lifetime. These findings demonstrate the validity of this strategy for precisely allocating the exciton harvesting in SEL WOLEDs.The coordination chemistry of cationic divalent pnictogen ligands, such as nitrenium and phosphenium, has been well-explored in recent years. However, corresponding studies of a heavier congener, stibenium ion, are rare. To better facilitate a Sb+-metal interaction, a tridentate P-Sb+-P ligand with two phosphine buttresses was designed and synthesized, and its coordination chemistry toward late transition metals was investigated. The stibenium ligand was delivered as an activated P(SbCl)P-AgOTf complex (2) that releases AgCl and the P-Sb+-P ligand upon the treatment with transition metals.  https://www.selleckchem.com/products/6-benzylaminopurine.html  Reacting 2 with Rh(I) and Ir(I) metals yielded the anticipated stibenium-transition-metal complexes [(Rh(COD)Cl)2(μ-PSb+P)] OTf ([3][OTf]) and [(Ir(COD)Cl)2(μ-PSb+P)] OTf ([4][OTf]). The M-Sb+-M bridging structure was confirmed by single-crystal X-ray crystallography, and the bonding situation was examined computationally. Theoretical studies revealed the presence of three-center delocalized M-Sb+-M bonding interactions in [3][OTf] and [4][OTf].DNA damage plays an important role in the regulation of gene expression and disease processes. The accurate measurement of DNA damage is essential to the discovery of potential disease biomarkers for risk assessment, early clinical diagnosis, and therapy monitoring. However, the low abundance, random location in genomic elements, diversity, and the incapability to specifically amplify the DNA damages hinder the accurate quantification of various DNA damages within human genomes. Herein, we demonstrate the integration of enzymatic labeling with single-molecule detection for sensitive quantification of diverse DNA damages. A significant advantage of our method is that only the damaged base-containing DNA sequence can be labeled by the biotin-conjugated deoxynucleotide triphosphate (biotin-dNTP) and separated from the normal DNAs, which greatly improves the detection specificity. In addition, high sensitivity can be achieved by the terminal deoxynucleotidyl transferase (TdT)-induced polymerization of multiple Alexa Fluor 488-labeled-deoxyuridine triphosphates (AF488-dUTPs) and the introduction of single-molecule detection.