https://www.selleckchem.com/products/q-vd-oph.html AIM/INTRODUCTION This study aimed to investigate the significance of zinc transporter 8 autoantibodies (ZnT8A) in identifying and characterizing autoimmune-mediated type 1 diabetes (T1D) in Japanese subjects. METHODS ZnT8A were determined in 324 patients with T1D, 191 phenotypic type 2 diabetes (T2D), and 288 healthy control subjects using bridging-type enzyme-linked immunosorbent assay (ELISA) in addition to autoantibodies to glutamic acid decarboxylase (GADA) and insulinoma-associated antigen-2 (IA-2A). RESULTS We set a cut-off value of 10.0U/mL, and twenty-five percent of the T1D patients had ZnT8A levels exceeding this level. The prevalence of ZnT8A was significantly higher in patients with acute-onset T1D than in those with slowly-progressive and fulminant T1D (P10 years (11.7% vs. 3.6%, P less then 0.05). Multivariate analysis revealed that ZnT8A positivity was independently associated with age at sampling and IA-2A positivity. CONCLUSION These results suggest that the bridging-type ZnT8A ELISA may provide a valuable additional marker for Japanese patients with T1D, which could, in turn, allow for an increase in the number of identifiable cases and differentiate clinical phenotypes. This article is protected by copyright. All rights reserved.In this work, a novel extraction technique based on the effervescence-assisted dispersion and magnetic recovery of attapulgite/polypyrrole sorbents was developed for determining the concentrations of five pyrethroids in honey samples. The magnetic nanoparticles were synthesized by a one-pot method. Several experimental parameters that affected the extraction efficiency, including the dispersion conditions, pH, ionic strength and desorption conditions, were investigated. Under optimal conditions, the calibration curves for the five pyrethroids in honey samples exhibited good linearity, with r2 values ranging from 0.9979 to 0.9990. The limits of detection varied between 0.21