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 Nutrients and oxygen are delivered mainly by blood vessels to nourish the cells and tissues in the body. Thus, biomaterials are processed by loading cytokines, such as vascular endothelial growth factors (VEGF), to facilitate angiogenesis in order to accelerate tissue regeneration. Nevertheless, the unpredictable biosecurity of exogenous cytokines is still a controversial issue for its clinical application. In this study, we constructed a kind of cytokine reservoir utilizing the binding affinity between heparin-like sulfate polysaccharide and endogenous growth factors. Two types of sulfated chitosan hydrogels, namely 6-O-sulfated chitosan (6-O-SCS) and 2-N,6-O-sulfated chitosan (2-N,6-O-SCS) hydrogels, were formed on the surface of the gelatin sponge matrix. The microstructure of the SCS-coated scaffolds is porous and interconnected, which is beneficial for cellular infiltration. Besides, human umbilical vein endothelial cells (HUVECs) can adhere and proliferate well on the surface of the scaffolds. Notably, sulfated chitosan-coated scaffolds exhibit an ability to capture VEGF in vitro & vivo, especially for the 2-N,6-O-SCS-coated scaffold. It is also verified by mice models that sulfated chitosan-coated scaffolds result in a concentrated VEGF microenvironment in specific domains as cytokine reservoirs and induce mass microvessels after implantation into subcutaneous tissues. As such, the sulfated chitosan-coated scaffolds served as VEGF reservoirs to accelerate angiogenesis and wound healing. This beneficial strategy may be applicable to in situ tissue regeneration by capturing more cytokines and promoting healing.A fluorescent amino poly(glycidyl methacrylate) (PGOHMA) was synthesized by atom transfer radical polymerization (ATRP) and post-polymerization. The obtained star polymer PGOHMA has eight polymer arms enriched with amine groups for gene delivery.  https://www.selleckchem.com/products/Bortezomib.html  PGOHMA showed much lower cytotoxicity and higher delivery efficiency, compared with the widely used commercial and previously reported gene carriers. Therefore, a new star-shaped fluorescent polymer was successfully synthesized and explored as an efficient gene carrier.This study reports a strategy of combining a Pt(iv) prodrug and a ruthenium nitrosyl (Ru-NO) donor into a single nanoplatform N-GQDs@Ru-NO-Pt@FA in which the platinum(iv) prodrug is conjugated onto a photoactivatable NO donor (Ru-NO) through a covalent bond and the nitric oxide-releasing platinum prodrug and folate groups are decorated on N-doped graphene quantum dots (N-GQDs). After cellular uptake of the nanoplatform, the platinum(iv) prodrug was reduced to an active anti-cancer Pt(ii) species inside the cancerous cells, and simultaneously, near-infrared (NIR) light illumination induced the release of NO, accompanied by a prominent photothermal effect. This nanoplatform is capable of targeting intracellular co-delivery of Pt(ii) and NO under 808 nm NIR light irradiation, accompanied by photothermal therapy, thereby leading to a significant synergistic therapeutic effect.Rapid and effective hemostasis for a noncompressible hemorrhage is the key to control bleeding and reduce mortality. Chitosan (CS) has been widely used as a popular hemostatic dressing; however, irregularly shaped wounds present in emergencies limit the performance of CS powder. To improve the hemostatic effect of CS, we modified it with poly(vinyl alcohol) (PVA), a fast-swelling sponge triggered by water. The novel synthetic PVA-CS was prepared by cross-linking PVA and CS during foaming and crosslinking reactions. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and X-ray diffraction were utilized to analyze the characteristics of PVA-CS. In vitro, the swelling ratio and blood clotting ability were evaluated in different groups with various weight ratios or degrees of deacetylation of the CS, and the cytocompatibility and cell attachment on the material were analyzed by human dermal fibroblast (HDF) cell testing. In vivo, the hemostatic effects were evaluated in Sprague-Dawley rats and Bama miniature pigs in a femoral artery hemorrhage model or gunshot wound experiment. PVA-CS presents robust mechanical strength, rapid water-triggered swelling and a fast absorption speed. As compared with gauze and PVA, which are widely used in first aid, PVA-CS sponges showed an improved blood clotting ability and increased blood cell and platelet adhesion and activation. The PVA-CS sponges also showed high biocompatibility in cell viability, cell proliferation and cell attachment bioassays. Furthermore, in vivo evaluation of the PVA-CS sponges revealed excellent hemostatic performance and enhanced wound healing with increased re-epithelialization and decreased granulation tissues. The results of this study strongly support the use of these composite sponges for noncompressible hemorrhage in acute trauma and ballistic injuries.Stem cell differentiation plays a significant role in tissue repair and regeneration.  https://www.selleckchem.com/products/Bortezomib.html  The interaction between stem cells and physical signals mediated by materials has significant influence on the fate of stem cells. The utilization of the stimulation originating from material physical properties to promote stem cell differentiation is being developed and has attracted much attention. However, it is difficult to induce electric signals into tissues noninvasively. In this study, piezoelectric nylon-11 nanoparticles (nylon-11 NPs) with uniform morphology were synthesized in mass production by a simple anti-solvent method. The prepared nylon-11 NPs possessed efficient piezoelectricity and high cytocompatibility. Fluorescent OPDA-coated nylon-11 NPs could image dental pulp stem cells (DPSCs) well, which demonstrated that nylon-11 NPs can be endocytosed easily by DPSCs. With the assistance of ultrasound, nylon-11 NPs could promote the osteogenic differentiation of DPSCs efficiently in a noninvasive way. Meanwhile, nylon-11 NPs could also promote the osteogenic differentiation of DPSCs to a certain extent. Therefore, piezoelectric nylon-11 NPs with the assistance of ultrasound will have enormous potential in tissue engineering, especially in stem cell fate regulation by noninvasive stimulation. This indicates that nanomaterial-mediated physical signals can regulate stem cell differentiation efficiently.