To investigate the regulatory mechanism of sevoflurane-induced neuronal apoptosis through analyzing the expression of glial cell-derived neurotrophic factor (GDNF) mediated by miR-133, sponged by long non-coding RNA (lncRNA) CDKN2B-AS1. An in vitro cell injury model was established by using different concentrations of sevoflurane and primary hippocampal neurons. Cell proliferation was detected by Cell Counting Kit-8 (CCK-8); caspase-3 and caspase-9 activities were detected by colorimetry, and apoptotic cells were determined by Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Fluorescence in situ hybridization (FISH) analysis was used to detect localized expression of CDKN2B antisense RNA 1 (CDKN2B-AS1), and dual-luciferase reporter assay was employed to verify the correlation of CDKN2B-AS1 and miR-133, and of miR-133 and GDNF. The expression of CDKN2B-AS1, miR-133, and GDNF mRNA in the cell injury model were measured by quantitative reverse transcription-polymerase chain reactioal apoptosis, and ease the toxic effect of sevoflurane on neural cells by acting as a sponge to adsorb miR-133. This study was designed to determine the effect of high-quality nursing intervention on negative emotions, quality of life and activities of daily living (ADL) of elderly patients with Parkinson's disease (PD). Totally 115 elderly PD patients treated in our hospital from March 2018 to September 2019 were selected as the research participants. According to different nursing intervention methods, they were divided into two groups. The research group (RG) (65 cases) received high-quality nursing intervention, while the control group (CG) (50 cases) received routine nursing intervention. https://www.selleckchem.com/products/disodium-r-2-hydroxyglutarate.html The adverse reactions, negative emotions, quality of life, ADL, PSQI, MDRSPD scores and nursing satisfaction were compared between the two groups. After nursing intervention, the incidence of adverse reactions in the RG was dramatically lower than that in the CG. Before nursing intervention, there was no marked difference in the scores of quality of life, ADL and MDRSPD between the two groups. But after nursing, those scores in the RG were markedly higher than those in the CG. Before nursing intervention, there was no remarkable difference in SAS, SDS and PSQI scores between both groups, but after that, the scores of the RG were obviously lower than those of the CG. After nursing intervention, the nursing satisfaction of patients in the RG was dramatically higher than that in the CG. High-quality nursing intervention for elderly PD patients can dramatically improve their negative emotions, quality of life and ADL, and promote recovery of motor function. High-quality nursing intervention for elderly PD patients can dramatically improve their negative emotions, quality of life and ADL, and promote recovery of motor function. For investigating Dbx2's expression in endometrial cancer (EC) and its effect on prognosis of patients with EC. A comparison was performed in the Cancer Genome Atlas (TCGA) database in terms of the expression profiling of EC and the survival data. To obtain differential expression genes (DEGs), Volcano plot and Venn analysis were adopted. DEGs function was performed by carrying out the GO annotation analysis (GO) and gene set enrichment analysis (GSEA). In clinical EC samples, PCR was applied to the verification of Dbx2's expression. Dbx2 was a downregulated expression in tumor tissues. Dbx2 can have a poor prognosis role in EC by regulating the apoptotic signaling pathway and the immune pathway. Lower expression of Dbx2 was related to lymph node metastasis and FIGO stage. Dbx2 is downregulated in endometrial cancer, which serves as a biomarker to predict poor prognosis. Dbx2 is downregulated in endometrial cancer, which serves as a biomarker to predict poor prognosis. To examine the effect of autophagy on cerebral damage caused by different models and test the hypothesis that its protection mechanism acts via inhibiting expression of neuroinflammatory mediators. Autophagy was induced by rapamycin treatment. Cerebral damage was induced using models of IL-6 treatment, oxygen glucose deprivation/reoxygenation (OGD/R) , and middle cerebral artery occlusion (MCAO) . The effect and mechanism of autophagy was examined and assessed in terms of cell viability, infarction size in brain tissue, neurological score, production of inflammatory mediators IL-1β and IL-6, transcription and protein expression of autophagy markers beclin-1 and LC-3II in different experimental groups. Autophagy triggered by rapamycin could protect neurons from IL-6-induced injury and astrocytes from OGD/R-induced injury and in rat brain tissue from MCAO . Autophagy significantly increased cell viability, attenuated cerebral infarction and improved neurological scores. It also inhibited production of the IL-1β and IL-6 and elevated the expression of beclin-1 and LC-3II. Autophagy can inhibit the inflammatory response and reduce cerebral I/R injury. There was a relationship between the extent of protection and (i) the level of the autophagic response, (ii) the stage of the cerebral I/R injury, and (iii) the time of intervention. Autophagy can inhibit the inflammatory response and reduce cerebral I/R injury. There was a relationship between the extent of protection and (i) the level of the autophagic response, (ii) the stage of the cerebral I/R injury, and (iii) the time of intervention. Type 2 diabetes (T2D) is a clinically common cardiovascular disease that can lead to kidney damage and adversely affect male fertility and sperm quality. Resveratrol (Res) is a natural product that has a wide range of effects in animals and cell models. This research is designed to observe the effect of resveratrol (Res) intervention on renal pathologic injury and spermatogenesis in mice with type 2 diabetes (T2D). Sixty healthy male SD mice without specific pathogens (SPF grade) were selected, and numbered by statistical software to randomize into control group (CG; n=20), model group (MG; n=20) and research group (RG; n=20). Mice in CG were given regular diet, while those in MG and RG were fed with high fat diet. Subsequently, RG was given Res intervention while MG received no treatment. Biochemical indexes [triglyceride (TG), total cholesterol (TC), fasting blood glucose (FBG), 24-hour urinary albumin excretion rate (24h-UAER)] of mice in the three groups before and after intervention were observed and recorded.