Further, the changes in the morphology of the material after functionalization were evaluated by scanning electron microscopy and atomic force microscopy. Finally, water contact angle measurement revealed manyfold increase in hydrophobicity after click polymerization. A video is also provided in the Supporting Information to show the application potential of this material for developing leather-like materials.Laser direct write (LDW) is a promising three-dimensional (3D) printing technology for creating proteinaceous microstructures in which the proteins retain their original function, enabling the manufacture of complex biomimetic 3D microenvironments and versatile enhancement of medical microdevices. A photoactivator has commonly been used to date in the laser direct write of proteins to enhance the cross-linking process. However, incomplete conversion results in photoactivator molecules remaining trapped inside the protein microstructure, causing their gradual leaching and subsequent undesirable effect on biological applications. Here, we demonstrate the 3D fabrication of microstructures made of pure serum albumin protein using photoactivator-free fabrication, confirmed by Raman data. For the first time, acid-catalyzed hydrolysis of the created structures provides evidence that chemical cross-links are induced by exposure to femtosecond laser irradiation. The diversity of the biomaterial protein available for the precursors for LDW offers capability of the fabrication of complex biomimetic 3D microenvironments and biochip applications.The clinical therapeutics for nerve tissue regeneration and functional recovery after spinal cord injury (SCI) are very limited because of the complex biological processes and inhibitory microenvironment. Advanced biomaterials are highly desired to avoid severe secondary damage and provide guidance for axonal regrowth. Multichannel nanofibrous scaffolds were modified with gelatin and cross-linked by genipin. The gelatin-coated nanofibers exhibited strong binding affinity with neurotrophin-3, which underwent a well-controlled release and highly promoted neuronal differentiation and synapse formation of the seeded neural stem cells.  https://www.selleckchem.com/products/shield-1.html  The nanofibrous scaffolds fabricated by combinatorial biomaterials were implanted into complete transected spinal cords in rats. Not only were the inflammatory responses and collagen/astrocytic scar formation limited, but the functional neurons and remyelination were facilitated postsurgery, leading to highly improved functional restoration. This nanofibrous scaffold with high specific surface area can be easily modified with biomolecules, which was proven to be effective for nerve regeneration after transected SCI, and provided a springboard for advanced scaffold design in clinical applications.CO-releasing molecules (CORMs) have been widely studied for their anti-inflammatory, antiapoptotic, and antiproliferative effects. CORM-3 is a water-soluble Ru-based metal carbonyl complex, which metallates serum proteins and readily releases CO in biological media. In this work, we evaluated the anti-inflammatory and wound-healing effects of gold nanoparticles-CORM-3 conjugates, AuNPs@PEG@BSA·Ru(CO)x, exploring its use as an efficient CO carrier. Our results suggest that the nanoformulation was capable of inducing a more pronounced cell effect, at the anti-inflammatory level and a faster tissue repair, probably derived from a rapid cell uptake of the nanoformulation that results in the increase of CO inside the cell.In situ-forming hydrogels present a promising approach for minimally invasive cell transplantation and tissue regeneration. Among prospective materials, hyaluronic acid (HyA) has displayed great potential, owing to its inherent biocompatibility, biodegradation, and ease of chemical modification. However, current studies in the literature use a broad range of HyA macromer molecular weights (MWs) from less then 100 kDa to 1 MDa with no consensus regarding an optimal MW for a specific application. We investigated the effects of different HyA macromer MWs on key biophysical properties of semisynthetic hydrogels, such as viscosity, gelation time, shear storage modulus, molecular diffusion, and degradation. Using higher-MW HyA macromers leads to quicker gelation times and stiffer, more stable hydrogels with smaller mesh sizes. Assessment of the potential for HyA hydrogels to support network formation by encapsulated vascular cells derived from human-induced pluripotent stem cells reveals key differences between HyA hydrogels dependent on macromer MW. These effects must be considered holistically to address the multifaceted, nonmonotonic nature of HyA MW on hydrogel behavior. Our study identified an intermediate HyA macromer MW of 500 kDa as providing optimal conditions for a readily injectable, in situ-forming hydrogel with appropriate biophysical properties to promote vascular cell spreading and sustain vascular network formation in vitro.A novel nonreleasing antibacterial hydrogel dressing with good reusability was prepared by polyethylene glycol dimethacrylate, N,N-methylene-bis-acrylamide, methyl methacrylate, 1-vinyl-3-butylimidazolium, and acrylamide. The ionic liquid of 1-vinyl-3-butylimidazolium was polymerized in the hydrogel to endow the hydrogel dressing with an antibacterial property. The successful synthesis of the hydrogel dressing was proven by FT-IR spectroscopy and energy-dispersive spectrometry. The morphology of the hydrogel was confirmed to be a porous and interconnected network structure by scanning electron microscopy. The resultant hydrogel dressing showed many desirable features, such as a good protein adsorption property and repeatable adhesiveness as well as excellent mechanical properties. Remarkably, the hydrogel dressing displayed broad antibacterial activity against bacteria Staphylococcus aureus and Escherichia coli and fungal Candida albicans. In addition, the hydrogel dressing applied locally on wounded skin of rats could effectively avoid early infection and further accelerate wound healing. The results indicated that the nonreleasing antibacterial hydrogel had potential application in wound dressing.