In this study the effect of the carbon source on L-valine production kinetics using genetically modified E. coli was researched. Glucose, lactose, Whey (W) and deproteinized whey (DW) were tested as carbon sources, keeping the carbon/nitrogen (C/N) ratio constant. Biomass generation and substrate consumption were modeled with Contois and Mass Conservation models, respectively, whereas Mass Conservation Balance and Luedeking-Piret models were used for product obtaining. Results showed that L-valine production is partially associated to growth, with values of 0.485 g L-valine/(g dry cell weight.h), and a product loss effect at a specific rate (β) of 0.019 g L-valine/(g dry cell weight.h) with W. The yield of this product increased 36 % using W concerning glucose or lactose as carbon sources. On the other hand, Mass Balance and Luedeking-Piret models adjust properly to experimental data (R2 >0.90). In conclusion whey is a promising substrate for obtaining L-valine using genetically-modified E. coli.The latest addition to the family of Coronaviruses, SARS-CoV-2, unleashed its wrath across the globe. The outbreak has been so rapid and widespread that even the most developed countries are still struggling with ways to contain the spread of the virus. The virus began spreading from Wuhan in China in December 2019 and has currently affected more than200 countries worldwide. Nanotechnology has huge potential for killing viruses as severe as HIV, herpes, human papilloma virus, and viruses of the respiratory tract, both inside as well as outside the host. Metal-nanoparticles can be employed for biosensing methodology of viruses/bacteria, along with the development of novel drugs and vaccines for COVID-19 and future pandemics. It is thus required for the nanoparticles to be synthesized quickly along with precise control over their size distribution. In this study, we propose a simple microfluidic-reactor-platform for in-situ metal-nanoparticle synthesis to be used against the pandemic for the development of preventive, diagnostic, and antiviral drug therapies. The device has been fabricated using a customized standard photolithography process using a simple and cost-effective setup. The confirmation on standard silver and gold metal nanoparticle formation in the microfluidic reactor platform was analysed using optical fiber spectrophotometer. This novel microfluidic platform provides the advantage of in-situ synthesis, flow parameter control and reduced agglomeration of nanoparticles over the bulk synthesis due to segregation of nucleation and growth stages inside a microchannel. The results are highly reproducible and hence scaling up of the nanoparticle production is possible without involving complex instrumentation.Endocrine disruptors are compounds that alter the functioning of the endocrine system of humans and wildlife. A large number of chemicals have been identified as EDs and humans can be exposed to them through dietary and/or environmental exposure (air, water, soil). At international level, scientific discussion on the topic of EDs focuses on the issue of setting out the scientific criteria according to which the key properties of these substances that render them EDs are determined. Regulatory action in EU has been impacted by the aforementioned discussion and, in particular, Regulation 2017/2100 and Regulation 2018/605 have been issued. However, these scientific criteria do not constitute a complete framework for the detection of EDs and, therefore, their adoption does not entail a fully effective human health protection. Moreover, glyphosate-based herbicides (GBH), are the most widely used pesticides worldwide. The glyphosate controversy turned the spotlight on pesticide regulation in the EU. The disagreement between IARC and regulatory evaluations of EFSA/ECHA has received great attention of citizens, organizations and stakeholders, as a result of methodological differences in the evaluation of the available evidence have been identified. This paper outlines the glyphosate controversy, following an overview of the EU EDs Regulation and pesticide legislation.Pesticides are commonly used in agriculture to enhance crop production and control pests. Therefore, pesticide residues can persist in the environment and agricultural crops. Although modern formulations are relatively safe to non-target species, numerous theoretical and experimental data demonstrate that pesticide residues can produce long-term negative effects on the health of humans and animals and stability of ecosystems.  https://www.selleckchem.com/products/marimastat.html  Of particular interest are molecular mechanisms that mediate the start of a cascade of adverse effects. This is a review of the latest literature data on the effects and consequences of contamination of agricultural crops by pesticide residues. In addition, we address the issue of implicit risks associated with pesticide formulations. The effects of pesticides are considered in the context of the Adverse Outcome Pathway concept.A controlled experimental model of exposure to aerosols particularly for cement dust was recently invented in a study from the laboratory that found high serum levels of heavy metals, decrease gastrointestinal motility, and altered hematological variables in cement dust exposed rats. However, reproducibility was not considered. This work aims at standardizing the model and investigating preliminary toxicological indicators. Thirty male rats used in this study were divided into 3 groups (n = 10). Group 1; control, while groups 2 and 3 were exposed to cement dust for 14 days and 28 days respectively. We assessed clinical signs of toxicity, tissue heavy metal concentration, histopathological, and body weight (BW) changes. We observed poor movement coordination, abnormal posture, cephalic fur loss. Evidence of ischemia and fibrotic pneumoconiosis were grossly observed in the lungs of the exposed groups. There was a significant increase in tissue level of heavy metals with pulmonary and gastric heavy metal content showing a trendy relationship during the period of the exposure as the value of Lead, Chromium, Cadmium, Iron, Calcium, and Nickel increased by nearly similar percentages in both tissues. Organs weights increased; the 14-day exposed (198 ± 31; 168 ± 22) and 28-day exposed (198 ± 22; 187 ± 26) groups had significantly reduced body weight at the first and second weeks of exposure compared to the control group (265 ± 26; 357 ± 40) respectively. Exposure to cement dust induced low bone density in the exposed rats (p  less then  0.05). Histopathological alterations include necrosis, inflammatory cellular infiltration, and alveolar hyperplasia suggestive of the proliferative response of pulmonary tissue to the dust. The operation of the standardized apparatus mimics a typical occupational exposure and the findings show that cement dust induces systemic toxicity via respiratory perturbation and body/organ weight discordance mediated by heavy metal bioaccumulation.