Photodynamic therapy (PDT) is an anticancer modality depicting an induced oxidative stress as the mechanism of action that ultimately culminates in cell death. The apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a key protein promoting bad prognostic in several cancer types. APE1/Ref-1 is able to regulate cell response to oxidative stress by two basic protein activities, including a reduction-oxidation-function and a DNA repair-function. Therefore, the efficacy of anticancer therapies is negatively affected by APE1-overexpression. Thus, here it was evaluated the potential of APE1-chemical inhibitors as sensitizers for PDT in two different cancer cell lines (A549 and HeLa cells). Both functions of APE1 were addressed using E3330 (redox-function) and CRT0044876 (DNA repair-function) molecules. A detailed cytotoxicity screening (cell viability, cell cycle kinetics, mitochondrial perturbation, and cell death) indicated HeLa cells as extremely sensitive (~ 3.5×) to the combination of PDT with E3330 when compared to A549 cells. The treatment using PDT with E3330 induced downregulation of APE1 as detected by Western Blot. The APE1's downregulation correlated to an increase of DNA fragmentation (17% and 66% in A549 and HeLa cells, respectively) and cell death rate (total 24% and 74% in A549 and HeLa cells, respectively) characterized by annexin V and 7-AAD markers as well as a considerable difference in superoxide detected in mitochondria (29% and 78% in A549 and HeLa cells, respectively).  https://www.selleckchem.com/products/mdivi-1.html  This study definitively detected an increase in PDT efficacy when APE1's redox function is dysregulated by E3330.Light plays an essential role in the outcome of plant-pathogen interactions. In this study, the effect of pre- and post-inoculation light quality on Botrytis cinerea disease development in strawberry leaves was investigated. Circadian variations in leaf susceptibility to Botrytis cinerea were tested by inoculating at 5 h, 12 h, or 16 h after the start of photoperiod. The effects of 4 h and/or 12 h of red or blue light combined with white light in a 16 h photoperiod on leaf susceptibility and metabolites were studied. Light presence significantly decreased leaf susceptibility to Botrytis irrespective of light quality. Leaf susceptibility to B. cinerea showed significant circadian variations. Susceptibility was highest in leaves inoculated 12 h after the start of photoperiod followed by incubation in the dark. When disease development took place under the light regime, the shorter the light period after inoculation the stronger the disease symptoms. Twelve hours of red light radiation in a 16 h photoperiod increased, while 12 h blue light radiation decreased leaf resistance, resulting in alterations in the circadian variation of leaf susceptibility. These alterations correlated with changes in leaf metabolites, such as hydrogen peroxide, total phenolics and hexoses. Overall, we conclude that twelve hours of red light followed by white light in a 16 h photoperiod efficiently increased leaf resistance to B. cinerea.Heavy metal contamination is becoming a global problem threatening human health. Heavy metal removal by engineered microbes by cellular adsorption and uptake is a promising strategy for treatment of heavy metal contamination. However, this strategy is confronted with limited heavy metal-capturing elements. In this study, we performed a transcription profiling-guided strategy for construction of heavy metal-capturing synthetic bacteria. Transcription profiling of a heavy metal-tolerating Cupriavidus taiwanensis strain revealed up-regulation of sulfur metabolism-related operons (e.g., iscSAU and moaEDAB) by Pb2+ and Cd2+. A synthetic Escherichia coli strain, EcSSMO, was constructed by design of a synthetic sulfur metabolism operon (SSMO) based on iscSAU/moaEDAB. Biochemical analysis and X-ray photoelectron spectroscopy (XPS) revealed that the synthetic bacteria had remodeled sulfur metabolism and enhanced heavy metal-tolerating capacity, with higher surviving EcSSMO cells than the surviving control cells Ec0 (not containing SSMO) at 50 mg/L of Pb2+ and Cd2+ (>92 % versus 40 % of both heavy metals even at 50 mg/L of Pb2+ and Cd2+. This study reveals emphasizes feasibility of transcription profiling-guided construction of synthetic organisms by large-scale remodeling metabolic network.This study proposes a design of a household waste collection system based on a two-stage procedure. First, the bin location-allocation problem is solved by selecting collection sites from a set of potential sites, and determining the type and number of bins at each selected collection site. Second, bin-to-bin waste collection routes are obtained for a fleet of homogeneous vehicles that are restricted by either work shift duration or vehicle capacity. Mixed integer linear programming (MILP) models are proposed for both stages, considering the particular characteristics of the problem. The models are applied to a real-world instance in the commune of Renca in Santiago, Chile. The results of first stage indicate an important preference for small bins since they have a lower unitary cost. Due to the large size of the real instance, a Large Neighborhood Search (LNS) heuristic is used in the second stage to find good feasible vehicle routing solutions in a reasonable period of time. The results for the routing phase suggest a larger number of routes in the morning work shift since these routes have shorter distances. The LNS heuristic presents a satisfactory behavior when compared to the MILP model with small instances. The proposed bin-to-bin household waste collection vehicle routing presents a more efficient solution than the existing door-to-door waste collection in the commune of Renca with respect to the total daily traveled distance and the average work shift duration. Finally, a sensitivity analysis is presented and discussed for both models.We report a 60-year-old male with fibrin-associated diffuse large B-cell lymphoma (fa-DLBCL) in left atrial myxoma. Echocardiography showed a mass (63 mm × 33 mm) in the left atrium. Histological inspection indicated fa-DLBCL on the surface of atrial myxoma incidentally, together with extensive fibrinous like exudation on myxoma surface. Malignant cells were localized in solid sheets and nests at the peripheral area of the fibrinous exudation which were positive for B-lineage markers (CD20+, CD79a+, PAX-5+) and in situ hybridization of EBV-encoded RNA (EBER). PCR amplification showed clonal rearrangement of immunoglobulin heavy chain (IgH) genes. The patient was still alive with no recurrence in the 35-month follow-up after surgery. We also did a detailed clinicopathological analysis and literature review, which indicated that fa-DLBCL was a heterogeneous entity.