Further studies on the hippocampal tissue of the rats revealed that Tanshinone IIA treatment significantly increased the activity of SOD and GSH-Px while the level of MDA decreased compared to the model group. Additionally, the relative expression of apoptosis-associated genes caspase-3, caspase-8, and caspase-9 and inflammation-associated genes IL-1β and IL-6 in Tanshinone IIA-treated rats was lower than that in model rats. Finally, we confirmed hippocampal neuron loss and apoptosis by Nissl staining and TdT-mediated dUTP Nick end labeling (TUNEL). Taken together, these data imply that Tanshinone IIA can ameliorate CCL2-induced learning memory and cognitive impairment by impacting oxidative stress, inflammation, and apoptosis. Tanshinone IIA may be a potential therapeutic agent for the treatment of HAND. Copyright © 2020 Yuan-jun Liao et al.TXLNA (taxilin alpha), a binding partner of the syntaxin family, was identified as a key factor in the coordination of intracellular vesicle trafficking and highly expressed in various tumor cells. However, the accurate relation between TXLNA and tumorigenesis and progression of pancreatic adenocarcinoma (PAAD) is still unclear. The present study was designed to examine the expression profile of TXLNA and explore its prognostic significance in PAAD patients and the possible molecular regulatory mechanism by analyzing a series of data from databases, including GEPIA, LOGpc, STRING, and GeneMANIA. The results indicate that TXLNA mRNA and protein were remarkably increased in PAAD tissues compared with normal pancreatic tissues. The high TXLNA expression was significantly correlated with superior overall survival (OS), disease-free interval (DFI), disease specific survival (DSS), and progression-free interval (PFI) for PAAD patients. In summary, high TXLNA expression could predict favourable OS, DFI, DSS, and PFI for PAAD patients, and it might be as potential prognostic biomarkers and targets for PAAD. Copyright © 2020 Shuangyu Lv et al.Aims/Hypothesis. The complications affecting the peripheral nervous system, associated with diabetes mellitus, have been the focus of considerable research. Comparably less research has focused upon the effect of diabetes upon the central nervous system. In this study, we investigate the effect of diabetes upon motor-neuron potentials evoked in the motor cortex of streptozotocin diabetic rats. Methods In this study, we investigated the cortical-evoked motor-neuron potentials in streptozotocin-induced diabetic rats. Cortical potentials were evoked using direct current stimulation to the motor cortex, and the resulting evoked potentials were recorded in the sciatic nerve. As voluntary movement consists of repeated activation of muscles, repeated stimulation trials were used to determine the effect of diabetes upon the animals' ability to recuperate between stimulations. Results Our findings showed that diabetes severely decreased the amplitude of cortical-evoked potentials and compromised the recuperation of motor neurons between activation. Conclusion/Interpretation.  https://www.selleckchem.com/products/gcn2ib.html  The reduced amplitude and weakened recuperation of diabetic motor neurons potentially may contribute to impaired transmission in motor pathways and thereby motor dysfunction. Copyright © 2020 Jesper Guldsmed Madsen et al.The logistic regression (LR) model for assessing differential item functioning (DIF) is highly dependent on the asymptotic sampling distributions. However, for rare events data, the maximum likelihood estimation method may be biased and the asymptotic distributions may not be reliable. In this study, the performance of the regular maximum likelihood (ML) estimation is compared with two bias correction methods including weighted logistic regression (WLR) and Firth's penalized maximum likelihood (PML) to assess DIF for imbalanced or rare events data. The power and type I error rate of the LR model for detecting DIF were investigated under different combinations of sample size, moderate and severe magnitudes of uniform DIF (DIF = 0.4 and 0.8), sample size ratio, number of items, and the imbalanced degree (τ). Indeed, as compared with WLR and for severe imbalanced degree (τ = 0.069), there were reductions of approximately 30% and 24% under DIF = 0.4 and 27% and 23% under DIF = 0.8 in the power of the PML and ML, respectively. The present study revealed that the WLR outperforms both the ML and PML estimation methods when logistic regression is used to evaluate DIF for imbalanced or rare events data. Copyright © 2020 Marjan Faghih et al.Background High-altitude headache (HAH) is the most common complication after high-altitude exposure. Hypoxia-inducible factor- (HIF-) related genes have been confirmed to contribute to high-altitude acclimatization. We aim to investigate a possible association between HIF-related genes and HAH in the Chinese Han population. Methods In total, 580 healthy Chinese Han volunteers were recruited in Chengdu (500 m) and carried to Lhasa (3700 m) by plane in 2 hours. HAH scores and basic physiological parameters were collected within 18-24 hours after the arrival. Thirty-five single nucleotide polymorphisms (SNPs) in HIF-related genes were genotyped, and linkage disequilibrium (LD) was evaluated by Haploview software. The functions of SNPs/haplotypes for HAH were developed by using logistic regression analysis. Results In comparison with wild types, the rs4953354 "G" allele (P=0.013), rs6756667 "A" allele (P=0.013), rs6756667 "A" allele (EPAS1, and rs6520015 "C" allele in PPARA (P=0.013), rs6756667 "A" allele (PPARA (P=0.013), rs6756667 "A" allele (EPAS1, and rs6520015 "C" allele in PPARA (P=0.013), rs6756667 "A" allele (. Conclusions EPAS1 and PPARA polymorphisms were associated with HAH in the Chinese Han population. Our findings pointed out potentially predictive gene markers, provided new insights into understanding pathogenesis, and may further provide prophylaxis and treatment strategies for HAH.EPAS1, and rs6520015 "C" allele in PPARA (. Copyright © 2020 Yang Shen et al.Coconut water is a highly nutritious liquid food which is a by-product of the desiccated coconut industry. Freeze concentration is the most suitable concentration method for coconut water since the low-temperature operation for concentration does not deteriorate the original quality of coconut water. Suspension freeze concentration (SFC) and progressive freeze concentration (PFC) are the available FC methods, and SFC is a complex and expensive method compared with PFC. PFC is a novel freeze concentration technique to concentrate liquid food by using a simple system. The limitation of PFC is the lower product yield than SFC, and to overcome the problem, the partial ice-melting technique can be used. A simple cylindrical apparatus was used for PFC which consists of a sample vessel, agitator system, and a cooling bath (at -23°C ± 2°C temperature). The final concentration of the liquid product was directly affected by the apparatus agitator speed and sample vessel dipping speed. PFC agitator speed of 290 rpm and dipping speed of 1.