https://www.selleckchem.com/products/p22077.html Furthermore, high HMs and EC reduced the numbers of soil-specific bacterial and fungal groups and drove the succession of certain groups that were highly resistant to increased HMs and EC. In addition, many bacterial and fungal groups exhibited different response patterns to each HM, implying that, in multiple HM-contaminated soils, HMs jointly shaped the microbial communities. PICRUSt analysis suggested that high HMs significantly decreased the total gene abundance and most KEGG modules in the soils. High EC and low pH significantly enhanced the abundances of several two-component system-, electron transfer-, and methanogenesis-related modules. We conclude that excessive multiple HMs and EC principally repressed the microbial activity and severely drove the gradient succession of bacterial and fungal communities in the arid loess region. Huge amounts of wastewater that contain aromatic compounds such as benzene and phenols are discharged worldwide. Benzoate is a typical intermediate in the anaerobic transformation of those aromatic compounds. In this study, electrically conductive carbon-based materials of granulated activated carbon (GAC), multiwalled carbon nanotubes (MwCNTs), and graphite were evaluated for the ability to promote the benzoate degradation. The results showed that 82-93% of the electrons were recovered in CH4 production from benzoate. The carbon materials stimulated benzoate degradation in the sequence of GAC (5 g/L) > MwCNTs (1 g/L) ~ Graphite (0.1 g/L) > Control. Acetate was the only detected intermediate in the process of benzoate degradation. Taxonomic analyses revealed that benzoate was degraded by Syntrophus to acetate and H2, which were subsequently converted to methane by Methanosarcina (both acetoclastic methanogens and hydrogenotrophic methanogens) and Methanoculleus (hydrogenotrophic methanogens), and direct interspecies electron transfer (DIET) of Desulfovibrio and Methanosarcina. Thus,